CAJ | 학술논문

为探讨99 Tcm 标记的甘露糖基右旋糖苷类衍生物DPZM （Dextran-Amine-Pyrazole-Manose）用于前哨淋巴结显像的可行性，采用羰基锝标记了DPZM-4和DPZM-8，制得99 Tcm-（CO）3-DPZM 类配合物，考察标记物的体内分布及其在前哨淋巴结（Sentinel Lymph Node ，SLN）与次级淋巴结的放射性摄取，研究不同注射剂量对正常鼠的SLN摄取及体内分布的影响，并进行显像。结果表明，99 Tcm-DPZM-4和99 Tcm-DPZM-8的标记率均＞90％，配合物稳定性较好；在正常鼠体内，99 Tcm-（CO）3-DPZM 类衍生物的前哨淋巴结摄取率较高，给药后1h最高可达6．31％ ID，且持续浓集，给药后4h放射性摄取率为3．60％ ID，而在其他主要器官的摄取较少；另外，适当减少标记物的注射剂量（包括注射化学量和注射体积）可以提高SLN的摄取率和显像效果。99 Tcm-（CO）3-DPZM 的SLN摄取率和体内分布实验结果表明，该类化合物具有应用于SLN显像的潜在价值，值得进行进一步研究。
위탐토99 Tcm 표기적감로당기우선당감류연생물DPZM （Dextran-Amine-Pyrazole-Manose）용우전초림파결현상적가행성，채용탄기득표기료DPZM-4화DPZM-8，제득99 Tcm-（CO）3-DPZM 류배합물，고찰표기물적체내분포급기재전초림파결（Sentinel Lymph Node ，SLN）여차급림파결적방사성섭취，연구불동주사제량대정상서적SLN섭취급체내분포적영향，병진행현상。결과표명，99 Tcm-DPZM-4화99 Tcm-DPZM-8적표기솔균＞90％，배합물은정성교호；재정상서체내，99 Tcm-（CO）3-DPZM 류연생물적전초림파결섭취솔교고，급약후1h최고가체6．31％ ID，차지속농집，급약후4h방사성섭취솔위3．60％ ID，이재기타주요기관적섭취교소；령외，괄당감소표기물적주사제량（포괄주사화학량화주사체적）가이제고SLN적섭취솔화현상효과。99 Tcm-（CO）3-DPZM 적SLN섭취솔화체내분포실험결과표명，해류화합물구유응용우SLN현상적잠재개치，치득진행진일보연구。
In order to explore the potential application of 99 Tcm labeled mannosylated dextran conjugates with amine-pyrazole group (Dextran-amine-pyrazole-mannose ,DPZM ) as senti-nel lymph node (SLN ) imaging agent , 99 Tcm labeled DPZM conjugates (DPZM-4 and DPZM-8) were prepared through [99 Tcm (CO)3 ]+ precursor synthesized from Isolink kit , and the in-vivo biological behaviors of 99 Tcm-(CO )3-DPZM conjugates were investigated . The labeling yields of 99 Tcm-(CO)3-DPZM conjugates were above 90% ,and the labeled con-jugates were relatively stable in vitro and in vivo .The biodistribution results demonstrated that 99 Tcm-(CO)3-DPZM conjugates were accumulated mainly at injection sites and SLNs , the highest uptake in SLN was found to be 6 .31% ID 1 hp .i .and no significant uptakes were found in major organs such as liver and spleen .The biodistribution of these conjugates was also sensitive to the injected dosage ,the uptake of SLN and PE% increased while the injected dosage decreased .The results of SPECT imaging study corresponded to those of biodistribution study ,SLN can be clearly visualized and better images could be obtained at lower dosages .the high SLN uptake and PE% along with low radioactivity background of 99 Tcm labeled DPZM conjugates showed promise for SLN imaging and detection .