CAJ | 학술논문

为探讨枇杷[Eriobotrya japonica (Thunb.) Lindl.]果皮在高温强光胁迫下的蛋白质组分变化，采用蛋白质组学方法分析了果实日灼抗性差的枇杷种质‘WDYDB’果皮蛋白质对高温强光胁迫的应答反应。结果表明，在自然高温强光胁迫与遮光处理(对照)下，枇杷果皮蛋白质双向电泳图谱中表达量差异在2倍以上的蛋白点共有31个；通过MALDI-TOF-TOF/MS质谱分析成功鉴定出26个差异蛋白点，包括11个下调蛋白和15个上调蛋白。根据这些蛋白功能，可将其分为防御应答、碳水化合物和能量代谢、光合作用、其它等4类蛋白。同时，对这些蛋白质在高温强光胁迫下的功能和作用进行了讨论。这些差异蛋白质参与了枇杷对高温强光胁迫的响应。
위탐토비파[Eriobotrya japonica (Thunb.) Lindl.]과피재고온강광협박하적단백질조분변화，채용단백질조학방법분석료과실일작항성차적비파충질‘WDYDB’과피단백질대고온강광협박적응답반응。결과표명，재자연고온강광협박여차광처리(대조)하，비파과피단백질쌍향전영도보중표체량차이재2배이상적단백점공유31개；통과MALDI-TOF-TOF/MS질보분석성공감정출26개차이단백점，포괄11개하조단백화15개상조단백。근거저사단백공능，가장기분위방어응답、탄수화합물화능량대사、광합작용、기타등4류단백。동시，대저사단백질재고온강광협박하적공능화작용진행료토론。저사차이단백질삼여료비파대고온강광협박적향응。
In order to understand the changes in protein components of loquat [Eriobotrya japonica (Thunb.) Lindl.] under high temperature and strong light stress, the responses of proteins in loquat ‘WDYDB’ peels to stress were analyzed by using proteomic approaches, which fruits were sensitive to sunburn. The results showed that there were 31 protein dots with differential expression of more than two times on dimensional electrophoresis profiles, as compared between treatments of high temperature and strong light stresses and shading (Control). Among them, twenty-six differential proteins were identified by MALDI-TOF-TOF/MS analysis, including 11 down-regulated and 15 up-regulated proteins. These proteins could be divided into 4 categories according to their functions, including defense response, carbohydrate and energy metabolism, photosynthesis and other protein. The functions and roles of these proteins under high temperature and strong light stress were discussed. Therefore, it was suggested that these differential proteins could involve in responses to high temperature and strong light stresses.