CAJ | 학술논문

目的：探讨糖尿病机械性痛（DMA）模型大鼠中P2X3受体（P2X3R）的时空表达变化。方法腹腔注射链脲菌素（ STZ）建立大鼠DMA模型。采用von Frey细丝法测定DMA大鼠机械性痛阈，在不同时间点取腰髓4～5（ L4～5）节段的脊髓背角（ SDH）和背根神经节（ DRG）以及足底皮肤，通过免疫荧光观察P2X3R阳性产物的表达变化。进一步Western blotting检测SDH和DRG中P2X3R蛋白的变化。结果与对照组（ CON组）相比，注射STZ 7d后大鼠机械性痛阈显著降低，在14d时达到最低并持续至28d（ P＜0．05）。免疫荧光和Western blotting结果均显示L4～5节段DRG中P2X3R表达在14d和21d时显著增多，与CON组相比差异有统计学意义（P＜0．05），28d时恢复至CON组水平；而在SDH及皮肤中，P2X3R表达上调出现在21d和28d（P＜0．05）。结论在STZ诱导的DMA大鼠机械性痛的不同时程中，DRG、SDH和皮肤中P2X3R的表达均呈现与痛敏变化几乎平行的变化趋势，但后两者比前者的变化在时间上略晚。这些结果说明P2X3R可能在DMA机械性痛敏的维持阶段起重要作用。
목적：탐토당뇨병궤계성통（DMA）모형대서중P2X3수체（P2X3R）적시공표체변화。방법복강주사련뇨균소（ STZ）건립대서DMA모형。채용von Frey세사법측정DMA대서궤계성통역，재불동시간점취요수4～5（ L4～5）절단적척수배각（ SDH）화배근신경절（ DRG）이급족저피부，통과면역형광관찰P2X3R양성산물적표체변화。진일보Western blotting검측SDH화DRG중P2X3R단백적변화。결과여대조조（ CON조）상비，주사STZ 7d후대서궤계성통역현저강저，재14d시체도최저병지속지28d（ P＜0．05）。면역형광화Western blotting결과균현시L4～5절단DRG중P2X3R표체재14d화21d시현저증다，여CON조상비차이유통계학의의（P＜0．05），28d시회복지CON조수평；이재SDH급피부중，P2X3R표체상조출현재21d화28d（P＜0．05）。결론재STZ유도적DMA대서궤계성통적불동시정중，DRG、SDH화피부중P2X3R적표체균정현여통민변화궤호평행적변화추세，단후량자비전자적변화재시간상략만。저사결과설명P2X3R가능재DMA궤계성통민적유지계단기중요작용。
Objective To investigate the spatio-temporal expression of P2X3 receptor ( P2X3R) in rats with diabetic mechanical allodynia ( DMA ) .Methods DMA model in rats was induced by intraperitoneal injection of streptozocin ( STZ) .The von Frey filaments were applied to identify the changes of the paw withdrawal threshold ( PWT) in DMA rats.Immunofluorescence was employed to detect the spatio-temporal expression of P2X3R in the spinal dorsal horn (SDH), dorsal root ganglion (DRG) and hind paw skin on different time points after intraperitoneal injection of STZ , respectively.The protein expression of P2X3R in SDH and DRG was further semi-quantitatively analyzed by Western blotting.Results Compared with control group , intraperitoneal injection STZ induced significant mechanical allodynia indicated by the reduced PWT from 7 days, and which reached the peak on 14d and maintained to 28days (P<0.05). The expression of P2X3R in DRG neurons was significantly increased on 14 days and 21 days (P<0.05), while that in SDH and skin was markedly increased on 21 days and 28 days, compared with control group (P<0.05).Conclusion With the progress of DMA, the expression of P2X3R was significantly increased in the SDH, DRG and skin, which was almost parallel with the mechanical allodynia , but the changes in SDH and skin were 1 week later than that in DRG .These results suggest that P2X3R may play a key role in the maintenance of the DMA .