CAJ | 학술논문

目的：探讨NonO蛋白在丁酸钠诱导小鼠红白血病（ MEL）细胞向红细胞系分化过程中的表达变化。方法利用联苯胺染色观察丁酸钠诱导MEL细胞红细胞系分化情况，通过Western blotting、免疫细胞化学检测NonO蛋白在丁酸钠诱导MEL细胞分化的过程中表达量的变化，PCR技术检测NonO基因在RNA水平上的表达变化，免疫细胞化学技术检测NonO蛋白在MEL细胞中的定位。结果发现NonO在丁酸钠诱导MEL细胞向红细胞系分化过程中在基因水平和蛋白水平上均上调表达，NonO蛋白在MEL细胞中定位于细胞质和细胞核。结论NonO蛋白与丁酸钠诱导MEL细胞分化密切相关。
목적：탐토NonO단백재정산납유도소서홍백혈병（ MEL）세포향홍세포계분화과정중적표체변화。방법이용련분알염색관찰정산납유도MEL세포홍세포계분화정황，통과Western blotting、면역세포화학검측NonO단백재정산납유도MEL세포분화적과정중표체량적변화，PCR기술검측NonO기인재RNA수평상적표체변화，면역세포화학기술검측NonO단백재MEL세포중적정위。결과발현NonO재정산납유도MEL세포향홍세포계분화과정중재기인수평화단백수평상균상조표체，NonO단백재MEL세포중정위우세포질화세포핵。결론NonO단백여정산납유도MEL세포분화밀절상관。
Objective To study NonO protein expression changes in murine erythroleukemia ( MEL ) cell differentiation induced by sodium butyrate .Methods Benzidine staining was used to test sodium butyrate-induced erythroid differentiation of MEL cells .We detected NonO protein expression changes in MEL cell differentiation induced by sodium butyrate and NonO protein localization in MEL cells by Western blotting and immunocytochemistry .Furthermore , we applied PCR technique to detect NonO RNA expression in differentiation process .Results We found that NonO protein was upregulated at gene and protein levels in the erythroid differentiation process of MEL cells induced by sodium butyrate and located in cytoplasm and nucleus in MEL cells .Conclusion These results show that NonO protein is closely related with MEL cell differentiation induced by sodium butyrate , which may provide important clues for further study of the mechanism of leukemia .