CAJ | 학술논문

目的：利用二甲基亚砜（ DMSO）诱导小鼠红白血病（ MEL）细胞分化，系统地分析和鉴定诱导分化不同时间的差异蛋白质组学。方法 DMSO诱导MEL细胞分化，通过联苯胺染色、MTT比色法和Ter119免疫荧光等实验检测细胞分化的比率和细胞的活力，并利用双向电泳耦联质谱技术，结合生物信息学分析诱导MEL细胞分化过程中差异表达的蛋白质。结果1．2％DMSO诱导MEL细胞分化，分别培养0h、6h、12h、24h、36h、48h、72h、96h、120h，利用双向电泳和质谱分析，共鉴定出87种蛋白质。1．2％ DMSO作用MEL细胞120h后，细胞诱导分化率达到67％。MTT实验显示，1．0％、1．2％、1．4％的DMSO对MEL细胞的活力无明显的抑制作用。结论 DMSO对MEL细胞有诱导分化作用，但无明显地抑制细胞增殖。87种双向电泳差异蛋白质按功能可分为12类，比例最大的前3类分别是：占41％的酶类蛋白、15％的结构蛋白、13％的调节蛋白。
목적：이용이갑기아풍（ DMSO）유도소서홍백혈병（ MEL）세포분화，계통지분석화감정유도분화불동시간적차이단백질조학。방법 DMSO유도MEL세포분화，통과련분알염색、MTT비색법화Ter119면역형광등실험검측세포분화적비솔화세포적활력，병이용쌍향전영우련질보기술，결합생물신식학분석유도MEL세포분화과정중차이표체적단백질。결과1．2％DMSO유도MEL세포분화，분별배양0h、6h、12h、24h、36h、48h、72h、96h、120h，이용쌍향전영화질보분석，공감정출87충단백질。1．2％ DMSO작용MEL세포120h후，세포유도분화솔체도67％。MTT실험현시，1．0％、1．2％、1．4％적DMSO대MEL세포적활력무명현적억제작용。결론 DMSO대MEL세포유유도분화작용，단무명현지억제세포증식。87충쌍향전영차이단백질안공능가분위12류，비례최대적전3류분별시：점41％적매류단백、15％적결구단백、13％적조절단백。
Objective To explore the differentially expressed proteins during erythroid differentiation .Methods The murine erythroleukemia ( MEL) cell were treated by DMSO , and the comparative proteomic was systematically analyzed and identified on different differentiating time points .ratio of cell differentiation and viability were detected by benzidine staining, MTT assay and Ter119 immunofluorescence.Using two-dimensional gel electrophoresis combined with mass spectrometry technology and bioinformatics analysis , we conducted a comparative proteomic analysis on MEL cells during the process of induced differentiation to screen and identify differential proteins .Results The MEL cells induced by 1.2%DMSO for 0 hour, 6hours, 12hours, 24hours, 36hours, 48hours, 72 hours, 96 hours, 120 hours were collected for proteomic analysis, by two-dimensional gel electrophoresis combined with mass spectrometry .A total of 87 kinds of proteins were successfully identified .MEL cells exposed to DMSO at a final concentration of 1.2% for 120 hours reached the highest differentiation rate of 67%.MTT assay showed that 1.0%, 1.2%, 1.4% DMSO had no inhibiting effect on cell vitality.Conclusion DMSO may induce MEL cells to differentiate and have no inhibiting effect on cell vitality .The 87 kinds of differentially expressed proteins from two-dimentional gel electrophoresis may be divided into twelve categories ;the most three parts are 41%enzyme protein, 15%structural protein and 13%regulatory protein.