CAJ | 학술논문

目的：观察雌性山羊颈胸神经节内神经元是否具备接受孕激素作用的条件。方法采用免疫组织化学SP法观察孕激素受体（ PR）在成年雌性山羊颈胸神经节的表达。结果雌性山羊颈胸神经节内神经元胞体均呈孕激素受体免疫反应阳性；卫星细胞、施万细胞、神经突起和神经纤维中也有较弱的着色。神经元的细胞膜和细胞质呈棕褐色为强阳性，而神经元的细胞核着色出现异质性：85．10％的神经元细胞核为强阳性，其中31．91％的核仁呈清亮的空泡，为阴性；而14．90％的神经元细胞核呈空泡状不着色，其中7．45％的核仁为强阳性。卫星细胞、施万细胞和神经纤维均呈淡黄色，为弱阳性。图像分析表明，神经元胞体PR的相对表达量与其他非神经成分相比差异性极显著（ P＜0．01）。结论在颈胸神经节内神经元是孕激素作用的主要靶细胞之一，提示孕激素有可能通过影响雌性山羊颈胸神经节内神经元的活动参与心脏功能的神经调节，而颈胸神经节上的PR可能作为一个网络节点协调孕激素对心脏功能的内分泌调节和自主神经对心脏活动的神经调节。
목적：관찰자성산양경흉신경절내신경원시부구비접수잉격소작용적조건。방법채용면역조직화학SP법관찰잉격소수체（ PR）재성년자성산양경흉신경절적표체。결과자성산양경흉신경절내신경원포체균정잉격소수체면역반응양성；위성세포、시만세포、신경돌기화신경섬유중야유교약적착색。신경원적세포막화세포질정종갈색위강양성，이신경원적세포핵착색출현이질성：85．10％적신경원세포핵위강양성，기중31．91％적핵인정청량적공포，위음성；이14．90％적신경원세포핵정공포상불착색，기중7．45％적핵인위강양성。위성세포、시만세포화신경섬유균정담황색，위약양성。도상분석표명，신경원포체PR적상대표체량여기타비신경성분상비차이성겁현저（ P＜0．01）。결론재경흉신경절내신경원시잉격소작용적주요파세포지일，제시잉격소유가능통과영향자성산양경흉신경절내신경원적활동삼여심장공능적신경조절，이경흉신경절상적PR가능작위일개망락절점협조잉격소대심장공능적내분비조절화자주신경대심장활동적신경조절。
Objective In order to explore whether neurons in the cervicothoracic ganglion of female goat were equipped with the condition for the role of progesterone .Methods Immunohistochemical SP method was used to detect the expression of progesteron receptor ( PR) in cervicothoracic ganglion of female goats .Results The result indicated that PR-immunoreactive-products were mainly present in the cell bodies of neurons , and satellite cells , Schwann cells , neurapophysis and nerve fiber also had weaker staining .The cell membrane and cytoplasm of neurons represented brown as strong positive ,but the nucleus of neurons showed the presence of heterogeneity , 85.10% neurons ’ nucleus was strong positive,of which 31.91%nucleolus being clear vacuoles were negative;14.90%nucleus displaying vacuolization had no shaining, of which 7.45%nucleolus was strong positive .There were weak positive and flaxen PR-immunoreactive-products in satellite cells, Schwann cells, neurapophysis and nerve fiber .Image analysis shows that PR of neurons was extremely significant compared with other non-nerve cells ( P <0.01 ) . Conclusion The result proved that the sympathetic postganglionic neurons in cervicothoracic ganglion of female goat were the one of main target cells of progesterone ,suggesting that progesterone may be involving in the neuroregulation of cardiac functional activities through affectinng the activities of neurons in the cervicothoracic ganglion of female goat ,and PR in the cervicothoracic ganglion may act as a network node to coordinate endocrine regulation of progesterone and neuroregulation of autonomic nerve on the cardiac functional activities .