CAJ | 학술논문

目的：观察创伤后应激障碍（ PTSD）大鼠海马神经元凋亡和Caspase-12表达的变化。方法采用单一延长应激（SPS）方法刺激大鼠建立PTSD模型，健康雄性Wistar大鼠随机分为SPS 1d、4d、7d组和正常对照组。应用透射电子显微镜观察PTSD大鼠海马神经元的形态学改变，采用免疫组织化学、免疫印迹法和RT-PCR技术检测海马神经元Caspase-12表达的变化。结果 SPS后，海马神经元发生了凋亡的形态学改变，Caspase-12的表达于刺激后4d最多。结论 PTSD激活凋亡因子Caspase-12启动海马神经元经内质网途径细胞凋亡，可能是PTSD患者海马萎缩的一个病理生理学基础。
목적：관찰창상후응격장애（ PTSD）대서해마신경원조망화Caspase-12표체적변화。방법채용단일연장응격（SPS）방법자격대서건립PTSD모형，건강웅성Wistar대서수궤분위SPS 1d、4d、7d조화정상대조조。응용투사전자현미경관찰PTSD대서해마신경원적형태학개변，채용면역조직화학、면역인적법화RT-PCR기술검측해마신경원Caspase-12표체적변화。결과 SPS후，해마신경원발생료조망적형태학개변，Caspase-12적표체우자격후4d최다。결론 PTSD격활조망인자Caspase-12계동해마신경원경내질망도경세포조망，가능시PTSD환자해마위축적일개병리생이학기출。
Objective To observe changes of apoptosis and Caspase-12 expression in hippocampus of the rat model of posttraumatic stress disorder ( PTSD ) .Methods Single prolonged stress ( SPS ) was used to duplicate a rat model of PTSD.Male Wistar rats were randomly divided into 1, 4, 7 days groups after exposure to SPS and a normal control group.Transmission electron microscopy was used to observe morphologic changes of the hippocampus neurons .The expression of Caspase-12 was detected by immunohistochemistry , Western blotting and RT-PCR.Results After SPS, the hippocampus neurons had characteristic morphologic changes of apoptosis , and the expression of Caspase-12 reached the peak at SPS 4d.Conclusion PTSD activates the ERS-mediated apoptosis in hippocampus via Caspase-12, which may be a pathophysiology base of the atrophy of the hippocampus in PTSD .