CAJ | 학술논문

应用Biolog技术研究了连续2年氮素与水分添加处理对贝加尔针茅草原土壤微生物功能多样性的影响。结果表明，水分添加能够减少氮肥对微生物的抑制作用，增强微生物的代谢活性和微生物对碳源的利用能力，水分添加与N15水平氮处理的交互作用显著提高了土壤微生物对碳源的利用能力和功能多样性。与对照相比，高氮处理（N100、N150、N200和N300）下微生物群落丰富度指数和均匀度指数降低，而优势度指数升高。氮素与水分添加处理不仅改变了微生物的功能多样性，也使微生物对碳源的利用模式发生转变。主成分分析表明，低氮肥与高氮肥处理下土壤微生物对碳源利用模式上存在较大差异，氮肥是主要影响因子。糖类、氨基酸类和代谢中间产物及次生代谢物是土壤微生物利用的主要碳源。土壤总有机碳、全氮、铵态氮、硝态氮含量与微生物群落功能多样性密切相关。
응용Biolog기술연구료련속2년담소여수분첨가처리대패가이침모초원토양미생물공능다양성적영향。결과표명，수분첨가능구감소담비대미생물적억제작용，증강미생물적대사활성화미생물대탄원적이용능력，수분첨가여N15수평담처리적교호작용현저제고료토양미생물대탄원적이용능력화공능다양성。여대조상비，고담처리（N100、N150、N200화N300）하미생물군락봉부도지수화균균도지수강저，이우세도지수승고。담소여수분첨가처리불부개변료미생물적공능다양성，야사미생물대탄원적이용모식발생전변。주성분분석표명，저담비여고담비처리하토양미생물대탄원이용모식상존재교대차이，담비시주요영향인자。당류、안기산류화대사중간산물급차생대사물시토양미생물이용적주요탄원。토양총유궤탄、전담、안태담、초태담함량여미생물군락공능다양성밀절상관。
The effects of nitrogen fertilizer (0,15,30,50,100,150,200,300 kg N/hm2 )and water on soil mi-crobial functional diversity in Stipa baicalensis steppe were explored for 2 years by the Biolog method.Com-pared with non-irrigation,water enhanced soil microbial metabolic activity and the utilization ability of carbon sources.The interaction of irrigation and the N15 level of fertilizer significantly increased the utilization ability of carbon source and functional diversity of soil microorganisms.Higher N fertilizer (N100 ,N150 ,N200 and N300 ) significantly reduced the richness and evenness index of microorganisms,but increased the dominance index. Irrigation and N fertilizer led to changes not only of functional diversity,but also of carbon source utilization mode and metabolic function of the microbial communities.Principal component analysis (PCA)showed that there was a very significant difference in the carbon source utilization mode of the microbial communities be-tween the lower fertilizer treatments and higher fertilizer treatments indicating that N fertilizer was the main impact factor.Carbohydrates,amino acids,intermediate metabolites,and secondary metabolites were the main carbon source utilized by the microbial communities.In addition,organic carbon,total N,and available N in soil had a close relationship to microbial functional diversity.