CAJ | 학술논문

为鉴定评价早熟禾幼苗抗旱性，将14个早熟禾品种的种子经消毒后播种在塑料花盆中，出苗后间苗，2～3个真叶定株，每盆留生长一致、分布均匀的幼苗15株，定期浇水，以保证幼苗的正常生长。待生长到3～4个真叶时进行干旱处理，依据 Michel公式计算不同水势所需的PEG-6000的量，然后加入PEG-6000溶液进行干旱处理，处理的水势梯度为0 MPa（即为对照），-0．3，-0．6，-0．9和-1．2 MPa。处理后每2 d进行称重补水以维持溶液浓度。设干旱处理与对照（正常浇水）两组，对14个早熟禾品种幼苗进行模拟干旱胁迫，4次重复。测定叶绿素（CHL）含量、丙二醛（MDA）含量、脯氨酸（Pro）含量、过氧化物酶（POD）以及超氧化物歧化酶（SOD）活性等生理生化指标。结果表明，随着干旱胁迫的加剧，早熟禾幼苗叶片CHL含量呈下降趋势；Pro 含量、MDA含量、SOD活性总体呈上升趋势；POD活性总体呈先降再升后降的趋势；利用隶属函数分析得出14个早熟禾品种的抗旱性强弱顺序依次为：3号恩托佩草地早熟禾＞10号细叶早熟禾＞1号优异草地早熟禾＞2号斗士草地早熟禾＝9号蓝钻草地早熟禾＞7号草地早熟禾＞11号草地早熟禾＞4号草地早熟禾＞5号瓦巴斯草地早熟禾＞8号早熟禾＝13号肯塔基草地早熟禾＞14号草地早熟禾＞6号莫诺波利草地早熟禾＝12号菲尔津草地早熟禾。
위감정평개조숙화유묘항한성，장14개조숙화품충적충자경소독후파충재소료화분중，출묘후간묘，2～3개진협정주，매분류생장일치、분포균균적유묘15주，정기요수，이보증유묘적정상생장。대생장도3～4개진협시진행간한처리，의거 Michel공식계산불동수세소수적PEG-6000적량，연후가입PEG-6000용액진행간한처리，처리적수세제도위0 MPa（즉위대조），-0．3，-0．6，-0．9화-1．2 MPa。처리후매2 d진행칭중보수이유지용액농도。설간한처리여대조（정상요수）량조，대14개조숙화품충유묘진행모의간한협박，4차중복。측정협록소（CHL）함량、병이철（MDA）함량、포안산（Pro）함량、과양화물매（POD）이급초양화물기화매（SOD）활성등생리생화지표。결과표명，수착간한협박적가극，조숙화유묘협편CHL함량정하강추세；Pro 함량、MDA함량、SOD활성총체정상승추세；POD활성총체정선강재승후강적추세；이용대속함수분석득출14개조숙화품충적항한성강약순서의차위：3호은탁패초지조숙화＞10호세협조숙화＞1호우이초지조숙화＞2호두사초지조숙화＝9호람찬초지조숙화＞7호초지조숙화＞11호초지조숙화＞4호초지조숙화＞5호와파사초지조숙화＞8호조숙화＝13호긍탑기초지조숙화＞14호초지조숙화＞6호막낙파리초지조숙화＝12호비이진초지조숙화。
To compare the drought resistance of bluegrass seedlings,the 14 varieties of Kentucky bluegrass seed were sowing after disinfection in the plastic pots,then thinned after emergence and fixed strains at 2 to 3 true leaf,left 1 5 uniform growth and distribution plants in every pot and regularly watering to ensure the normal growth of the seedlings.Drought stress trails were identified at 2 to 3 true leaf period by adding the PEG-6000 according to the Michel formula which are:0 MPa (control),-0 .3 ,-0 .6 ,-0 .9 ,-1 .2 MPa in the study. Then supplement water every two days to maintain the concentration.Two groups of drought treatment and control (normal watering)were set to simulate drought stress of 14 bluegrass cultivars and four replications were set by treatment.The physiological and biochemical index,such as chlorophyll (CHL),malondialdehyde (MDA)content,proline (Pro)content,peroxidase (POD)and superoxide dismutase (SOD)activity were measured and analyzed.The content of CHL was decreased;and the content of the Pro,MDA and SOD showed upward trend;the POD activity increased at first and then decreased and finally increase again in the bluegrass seedlings;Drought resistance of 14 bluegrass varieties were obtained by subordinate function the or-der is:The No.3 Storpy>No.10 Narrow leaf bluegrass>No.1 Meri>No.2 Geronimoi= No.9 Blue apphire>No.7 Kentucky bluegrass>No.11 Kentucky bluegrass>No.4 Kentucky bluegrass>No.5 Wabash>No.8 Bluegrass= No.13 Kentucky>No.14 Kentucky bluegrass>No.6 Monopoly= No.12 Fylking.