草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
4期
162-170
,共9页
李中林%郭开秀%周守标%郑和权%刘坤
李中林%郭開秀%週守標%鄭和權%劉坤
리중림%곽개수%주수표%정화권%류곤
马兰%光照强度%形态特征%生理指标%黄酮含量
馬蘭%光照彊度%形態特徵%生理指標%黃酮含量
마란%광조강도%형태특정%생리지표%황동함량
Kalimeris indica%light intensities%biological characteristics%physiological indexes%flavone con-tent
以马兰为实验材料,通过梯度遮光处理,研究不同光照强度(100%,62.29%和35.17%透光率)下不同处理时间对马兰形态、生理指标和黄酮类化合物的影响。结果显示,1)随着处理时间的延长,马兰节间长均呈增长趋势,但遮光抑制马兰的节间长度,尤其在透光率为62.29%时抑制更显著;在透光率为35.17%时能增强马兰的分枝能力,透光率为62.29%时作用不明显;遮光处理抑制了马兰的直立生长,在透光率为62.29%时对马兰株高产生显著抑制;在透光率为35.17%时更有利于马兰叶的长度、宽度和叶锯齿个数的增加,透光率为62.29%时作用不显著。2)透光率为35.17%时显著抑制叶片叶绿素a、b和叶绿素的总含量;随着处理时间的延长,O2-·含量呈先降后升再降的趋势,SOD活性和 MDA含量呈先降后升的趋势,POD活性呈先升后降的趋势;随着光照强度的减弱, POD活性和 MDA含量呈下降趋势,SOD活性呈先下降后升高趋势;在透光率为35.17%时CAT活性显著低于对照,抑制作用显著。3)随着光照强度的降低,黄酮含量在叶中呈下降趋势,根中呈上升趋势,透光率为62.29%时茎中含量最高,自然光照次之,透光率为35.17%时最低。
以馬蘭為實驗材料,通過梯度遮光處理,研究不同光照彊度(100%,62.29%和35.17%透光率)下不同處理時間對馬蘭形態、生理指標和黃酮類化閤物的影響。結果顯示,1)隨著處理時間的延長,馬蘭節間長均呈增長趨勢,但遮光抑製馬蘭的節間長度,尤其在透光率為62.29%時抑製更顯著;在透光率為35.17%時能增彊馬蘭的分枝能力,透光率為62.29%時作用不明顯;遮光處理抑製瞭馬蘭的直立生長,在透光率為62.29%時對馬蘭株高產生顯著抑製;在透光率為35.17%時更有利于馬蘭葉的長度、寬度和葉鋸齒箇數的增加,透光率為62.29%時作用不顯著。2)透光率為35.17%時顯著抑製葉片葉綠素a、b和葉綠素的總含量;隨著處理時間的延長,O2-·含量呈先降後升再降的趨勢,SOD活性和 MDA含量呈先降後升的趨勢,POD活性呈先升後降的趨勢;隨著光照彊度的減弱, POD活性和 MDA含量呈下降趨勢,SOD活性呈先下降後升高趨勢;在透光率為35.17%時CAT活性顯著低于對照,抑製作用顯著。3)隨著光照彊度的降低,黃酮含量在葉中呈下降趨勢,根中呈上升趨勢,透光率為62.29%時莖中含量最高,自然光照次之,透光率為35.17%時最低。
이마란위실험재료,통과제도차광처리,연구불동광조강도(100%,62.29%화35.17%투광솔)하불동처리시간대마란형태、생리지표화황동류화합물적영향。결과현시,1)수착처리시간적연장,마란절간장균정증장추세,단차광억제마란적절간장도,우기재투광솔위62.29%시억제경현저;재투광솔위35.17%시능증강마란적분지능력,투광솔위62.29%시작용불명현;차광처리억제료마란적직립생장,재투광솔위62.29%시대마란주고산생현저억제;재투광솔위35.17%시경유리우마란협적장도、관도화협거치개수적증가,투광솔위62.29%시작용불현저。2)투광솔위35.17%시현저억제협편협록소a、b화협록소적총함량;수착처리시간적연장,O2-·함량정선강후승재강적추세,SOD활성화 MDA함량정선강후승적추세,POD활성정선승후강적추세;수착광조강도적감약, POD활성화 MDA함량정하강추세,SOD활성정선하강후승고추세;재투광솔위35.17%시CAT활성현저저우대조,억제작용현저。3)수착광조강도적강저,황동함량재협중정하강추세,근중정상승추세,투광솔위62.29%시경중함량최고,자연광조차지,투광솔위35.17%시최저。
The effects of light intensity (100%,62.29%,35.17%of full light radiation)and different exposure times on biological characteristics,physiological indexes and flavone content of Kalimerisindica were studied. 1 )With increased treatment time,the internode-length of all treatment types showed an increasing trend,but shading inhibited K. indica’s internode-length elongation,especially at 62.29%of full light radiation.Shading stimulated K. indica’s branching ability at 35.17% of full light radiation,but this was not significant at 62.29% of full light radiation.Shading,especially light shading,inhibited erect growth of K. indica.Deeper shading (35.17% of full light radiation)was conducive to increased leaf length,width and sawtooth,but was not significant at 62.29% of full light radiation.2)Chlorophyll a,b and total chlorophyll content decreased at 35.17% of full light radiation.The content of O2 -· increased after an initial decrease and then decreased again with an increase of exposure time to light radiation.The activity of SOD and the MDA content increased after an initial decrease with time while the activity of POD showed the opposite trend.With a reduction of light in-tensity,the activity of POD and MDA content increased and the activity of SOD also increased after an initial decrease.At 35.17% of full light radiation,K. indica’s CAT activity was significantly lower than that of the control.3)With a decline of light intensity,the flavonoids content decreased in the leaves,increased in the roots and was highest in the stems at 62.29% of full light radiation.