CAJ | 학술논문

对白菜型冬油菜F2群体主要生理生化特性的变化进行分析，利用陇油7号和陇油9号杂交得到103个F2单株，对越冬率、幼苗习性、超氧化物歧化酶（SOD）等8个指标进行调查、测定。对所得数据进行方差分析、聚类分析、相关分析、通径分析。结果表明，除SOD活性外，其余各指标在F2群体中呈连续变异，分布频率大致接近正态分布，同时存在双向超亲分离现象。在聚类距离为3．58处，F2群体分为8个类群，群体间具有较大的遗传差异。相关分析表明所有指标与越冬率均有显著或极显著相关。各生理生化指标对越冬率的通径系数与相关系数方向均一致，但通径系数更有利于解释其本质，通过通径分析得到各指标对白菜型冬油菜越冬率相对重要性从大到小的顺序是：幼苗习性，SOD活性，MDA含量，游离脯氨酸含量，POD活性，CAT 活性，可溶性蛋白含量。幼苗习性对越冬率主要是通过直接影响起作用，影响率达79．8％，CAT 活性对越冬率的影响主要是通过间接影响起作用的，间接影响率达68．7％，而其余各指标对越冬率的影响均是通过直接和间接影响共同起作用的。这些结果可为白菜型冬油菜数量性状抗寒基因定位分析提供有用的信息。
대백채형동유채F2군체주요생리생화특성적변화진행분석，이용롱유7호화롱유9호잡교득도103개F2단주，대월동솔、유묘습성、초양화물기화매（SOD）등8개지표진행조사、측정。대소득수거진행방차분석、취류분석、상관분석、통경분석。결과표명，제SOD활성외，기여각지표재F2군체중정련속변이，분포빈솔대치접근정태분포，동시존재쌍향초친분리현상。재취류거리위3．58처，F2군체분위8개류군，군체간구유교대적유전차이。상관분석표명소유지표여월동솔균유현저혹겁현저상관。각생리생화지표대월동솔적통경계수여상관계수방향균일치，단통경계수경유리우해석기본질，통과통경분석득도각지표대백채형동유채월동솔상대중요성종대도소적순서시：유묘습성，SOD활성，MDA함량，유리포안산함량，POD활성，CAT 활성，가용성단백함량。유묘습성대월동솔주요시통과직접영향기작용，영향솔체79．8％，CAT 활성대월동솔적영향주요시통과간접영향기작용적，간접영향솔체68．7％，이기여각지표대월동솔적영향균시통과직접화간접영향공동기작용적。저사결과가위백채형동유채수량성상항한기인정위분석제공유용적신식。
Overwintering rates,seedling habits,SOD activity,and five other physio-biochemical indicators in 103 F2 populations of a winter rapeseed (Brassicarapa)cross between Longyou 7 and Longyou 9 were studied using variance analysis,correlation analysis,cluster analysis and path analysis.The frequency of all indicators (except SOD)activity in the F2 populations was approximately normally distributed but with a slight skew.A tremendous transgressive segregation for all indicators was observed in the populations.At a cluster distance of 3.58,F2 populations were divided into eight groups,with great genetic differences.Correlation analysis showed that all indicators had significant differences in overwintering rates.The path coefficient and correlation coefficient between all indicators and overwintering rate were consistent,but the explanation of their relation-ships were better explained by the path coefficient.Through path analysis,the relative importance in descend-ing order of B. rapa winter survival rate was:Seedling habits,SOD activity,MDA content,free proline con-tent,POD activity,CAT activity,and soluble protein content.Seedling habits directly impacted winter surviv-al rates,and the influence reached 79.8%,but CAT activity was mainly through indirect effects and the indi-rect affect rate was 68.7%,with the rest of the indexes co-acting through both direct and indirect effects. These results provide useful information for quantitative trait loci (QTL)mapping.