CAJ | 학술논문

本研究以831份狗牙根种质资源为试材，根据15个数量和质量性状，研究了狗牙根初级核心种质构建的方法。从总体取样规模（5％，10％，15％，20％，25％和30％）、组内取样比例（简单比例、对数比例、平方根比例和多样性比例）和取样方法（随机取样和聚类取样）3个层次探讨构建狗牙根初级核心种质的最佳取样策略，共形成48种取样策略。应用均值差异百分率、方差差异百分率、极差符合率和变异系数变化率4个参数来检验各取样策略的优劣。比较初始种质和初级核心种质两者基于主成分的样品分布图及性状间的相关系数，对构建的初级核心种质进行确认。结果表明，25％是最适宜的总体取样比例；4种组内取样比例中平方根比例优于简单比例、对数比例和多样性比例；2种取样方法中，聚类取样明显优于随机取样。最终确定25％的总体取样规模下，组内采用平方根比例和聚类抽样方法是构建狗牙根初级核心种质的最佳取样策略。根据获得的最佳取样策略最终在表型水平建立了包含208份种质的狗牙根初级核心种质，该种质可以作为整体资源的代表性样本，在狗牙根种质资源的充分开发和利用中发挥重要作用。
본연구이831빈구아근충질자원위시재，근거15개수량화질량성상，연구료구아근초급핵심충질구건적방법。종총체취양규모（5％，10％，15％，20％，25％화30％）、조내취양비례（간단비례、대수비례、평방근비례화다양성비례）화취양방법（수궤취양화취류취양）3개층차탐토구건구아근초급핵심충질적최가취양책략，공형성48충취양책략。응용균치차이백분솔、방차차이백분솔、겁차부합솔화변이계수변화솔4개삼수래검험각취양책략적우렬。비교초시충질화초급핵심충질량자기우주성분적양품분포도급성상간적상관계수，대구건적초급핵심충질진행학인。결과표명，25％시최괄의적총체취양비례；4충조내취양비례중평방근비례우우간단비례、대수비례화다양성비례；2충취양방법중，취류취양명현우우수궤취양。최종학정25％적총체취양규모하，조내채용평방근비례화취류추양방법시구건구아근초급핵심충질적최가취양책략。근거획득적최가취양책략최종재표형수평건립료포함208빈충질적구아근초급핵심충질，해충질가이작위정체자원적대표성양본，재구아근충질자원적충분개발화이용중발휘중요작용。
In this study,the method of constructing Cynodondactylon pre-core collection was studied based on 15 quantitative and qualitative traits from 831 accessions.An optimal sampling scheme was selected from 48 strategies which consisted of sampling proportions (5%,10%,15%,20%,25%,and 30%),sampling pro-portions within group (simple proportion,logarithmic proportion,square root proportion and genetic propor-tion)and individual selection methods within group (random selection and cluster selection)evaluating with four effective parameters including mean difference percentage,variance difference percentage,coincidence rate of range and changeable rate of variation coefficient.The optimal sampling scheme was also confirmed by com-paring the accessions distribution plotted by first two principal components,correlation coefficients of traits be-tween two collections.The result indicated that 25% was the optimum sampling proportions.The square root proportion was better than simple proportion,logarithmic proportion and genetic proportion.The cluster selec-tion was more suitable than random selection.At 25%sampling proportion,using square root proportion with-in group and cluster selection method within group is the most suitable method for constructing C. dactylon pre-core collection.The result conducted by PCA analysis showed the genetic structure and variation of 831 ac-cessions was well represented by the pre-core collection.The most important phenotypic correlations between traits which may be under the control of co-adapted gene complexes were also preserved by the pre-core collec-tion.Based on the optimized sampling strategy,the pre-core collection consisting 208 accessions of C. dactylon were established and will play an important role in the utilization of C. dactylon genetic resources.