CAJ | 학술논문

目的：通过检测鼠双微体2（ murine double minute 2，MDM2）在人乳腺癌中的表达，探讨其在乳腺癌发生发展中的作用。方法选取拟手术切除的70例乳腺癌患者，自乳腺癌癌灶中心非坏死部位及距肿瘤切缘5 cm以外的配对正常乳腺组织分别收集标本。采用实时荧光定量RT-PCR和免疫组化法检测乳腺癌组织中MDM2 mRNA 及其蛋白的表达水平，定量分析其表达与临床病理特征的关系。结果 MDM2在乳腺癌组织中的表达水平显著高于癌旁正常乳腺组织，差异有统计学意义（ P ＝0.001）。 MDM2 mRNA水平与肿瘤大小、分期、组织学类型、淋巴结转移和HER-2阳性表达有关（ P＜0.05），与雌、孕激素受体无关（ P＞0.05）；MDM2蛋白表达与肿瘤组织学类型、淋巴结转移和HER-2阳性表达有关（ P＜0.05），与肿瘤大小、分期、和雌、孕激素受体无关（ P＞0.05）。结论乳腺癌组织中MDM2表达明显升高，在乳腺癌的浸润与转移中具有重要作用。
목적：통과검측서쌍미체2（ murine double minute 2，MDM2）재인유선암중적표체，탐토기재유선암발생발전중적작용。방법선취의수술절제적70례유선암환자，자유선암암조중심비배사부위급거종류절연5 cm이외적배대정상유선조직분별수집표본。채용실시형광정량RT-PCR화면역조화법검측유선암조직중MDM2 mRNA 급기단백적표체수평，정량분석기표체여림상병리특정적관계。결과 MDM2재유선암조직중적표체수평현저고우암방정상유선조직，차이유통계학의의（ P ＝0.001）。 MDM2 mRNA수평여종류대소、분기、조직학류형、림파결전이화HER-2양성표체유관（ P＜0.05），여자、잉격소수체무관（ P＞0.05）；MDM2단백표체여종류조직학류형、림파결전이화HER-2양성표체유관（ P＜0.05），여종류대소、분기、화자、잉격소수체무관（ P＞0.05）。결론유선암조직중MDM2표체명현승고，재유선암적침윤여전이중구유중요작용。
Objective To investigate the expression of murine double minute 2 ( MDM2) in breast cancer and paired normal breast tissues and to explore the significance of MDM 2 in breast cancer tumorigenesis .Methods mRNA and protein expressions of MDM2 in human breast cancer were measured by quantitative real-time RT-PCR and immunohistochemical staining .The relationship between MDM2 expression and clinicopathologic characters were analyzed .Results MDM2 expression in breast cancer was higher than that in normal breast tissue (P =0.001).The mRNA expression level of MDM2 was correlated with tumor size, staging, lymph node status , differentiated degree and HER-2 status ( P<0.05 ) .MDM2 protein expression level was correlated with lymph node status , dif-ferentiated degree and HER-2 status ( P<0.05 ) .Conclusion MDM2 expression in human breast cancer increases significantly and plays a key role in the invasion and metastasis of human breast cancer .