中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2014年
7期
35-39
,共5页
张涛%陈斌%吕逸清%王鸿祥%胡凯%金炎%杨昊%冯坦%黄燕平%韩银发%王益鑫%黄翼然
張濤%陳斌%呂逸清%王鴻祥%鬍凱%金炎%楊昊%馮坦%黃燕平%韓銀髮%王益鑫%黃翼然
장도%진빈%려일청%왕홍상%호개%금염%양호%풍탄%황연평%한은발%왕익흠%황익연
白细胞精液症%瘦素%精子%细胞凋亡
白細胞精液癥%瘦素%精子%細胞凋亡
백세포정액증%수소%정자%세포조망
leukocytospermia%leptin%spermatozoa%apoptosis
目的：探索白细胞精液症患者治疗前后精液常规、精浆瘦素（Leptin）及精子凋亡率与正常人群的差异，并分析其相关性。方法随机选取2010年10月至2012年5月本院泌尿男科门诊的白细胞精液症患者80例（WBC组），另选取生育力及各项精液参数均正常的生育人群标本40份作为对照组，在患者治疗前及经治疗精液白细胞水平恢复正常后，采用计算机辅助精液分析系统（CASA）行精液常规检测、放射免疫法检测精浆Leptin、Annexin V+PI染色-流式计数法测定精子凋亡率。比较各参数的组间差异并分析其相关性。结果 WBC组治疗前精子浓度及活力均低于对照组（P＜0.05，P＜0.01），精子凋亡率显著高于对照组（P＜0.01），精浆Leptin水平高于对照组（P＜0.05），精浆Leptin水平与精子凋亡率之间存在正相关性（r=0.678，P＜0.05），经治疗后精子活力显著增加（P＜0.01），凋亡率降低（P＜0.05），精浆Leptin水平亦明显下降（P＜0.05）。结论治疗前白细胞精液症患者精子浓度、活力下降，而精浆Leptin水平和凋亡率均有增加，精浆Leptin与精子凋亡率之间存在正相关性。治疗后精浆Leptin与精子凋亡率均出现下调，精液常规各项参数明显改善，提示Leptin可能参与了过量白细胞诱发精子细胞凋亡的途径。
目的：探索白細胞精液癥患者治療前後精液常規、精漿瘦素（Leptin）及精子凋亡率與正常人群的差異，併分析其相關性。方法隨機選取2010年10月至2012年5月本院泌尿男科門診的白細胞精液癥患者80例（WBC組），另選取生育力及各項精液參數均正常的生育人群標本40份作為對照組，在患者治療前及經治療精液白細胞水平恢複正常後，採用計算機輔助精液分析繫統（CASA）行精液常規檢測、放射免疫法檢測精漿Leptin、Annexin V+PI染色-流式計數法測定精子凋亡率。比較各參數的組間差異併分析其相關性。結果 WBC組治療前精子濃度及活力均低于對照組（P＜0.05，P＜0.01），精子凋亡率顯著高于對照組（P＜0.01），精漿Leptin水平高于對照組（P＜0.05），精漿Leptin水平與精子凋亡率之間存在正相關性（r=0.678，P＜0.05），經治療後精子活力顯著增加（P＜0.01），凋亡率降低（P＜0.05），精漿Leptin水平亦明顯下降（P＜0.05）。結論治療前白細胞精液癥患者精子濃度、活力下降，而精漿Leptin水平和凋亡率均有增加，精漿Leptin與精子凋亡率之間存在正相關性。治療後精漿Leptin與精子凋亡率均齣現下調，精液常規各項參數明顯改善，提示Leptin可能參與瞭過量白細胞誘髮精子細胞凋亡的途徑。
목적：탐색백세포정액증환자치료전후정액상규、정장수소（Leptin）급정자조망솔여정상인군적차이，병분석기상관성。방법수궤선취2010년10월지2012년5월본원비뇨남과문진적백세포정액증환자80례（WBC조），령선취생육력급각항정액삼수균정상적생육인군표본40빈작위대조조，재환자치료전급경치료정액백세포수평회복정상후，채용계산궤보조정액분석계통（CASA）행정액상규검측、방사면역법검측정장Leptin、Annexin V+PI염색-류식계수법측정정자조망솔。비교각삼수적조간차이병분석기상관성。결과 WBC조치료전정자농도급활력균저우대조조（P＜0.05，P＜0.01），정자조망솔현저고우대조조（P＜0.01），정장Leptin수평고우대조조（P＜0.05），정장Leptin수평여정자조망솔지간존재정상관성（r=0.678，P＜0.05），경치료후정자활력현저증가（P＜0.01），조망솔강저（P＜0.05），정장Leptin수평역명현하강（P＜0.05）。결론치료전백세포정액증환자정자농도、활력하강，이정장Leptin수평화조망솔균유증가，정장Leptin여정자조망솔지간존재정상관성。치료후정장Leptin여정자조망솔균출현하조，정액상규각항삼수명현개선，제시Leptin가능삼여료과량백세포유발정자세포조망적도경。
Objective To detect the seminal Leptin level, sperm parameters and sperm apoptosis rate of leukocytospermia patients before and after treatment, and explore their correlation. Methods Total of 80 leukocytospermia patients(WBC group)were randomly recruited in the study and their sperm samples were collected before and after treatment. Other 40 normal sperm samples were collected as the controls. The sperm parameters were detected by computer assisted sperm analysis (CASA). The level of semen plasma Leptin was measured by RIA and the sperm apoptosis rate were assessed by AnnexinV/PI staining-FACS. Results Sperm concentration and viability in WBC group before treatment were all obviously lower than those of the control (P<0.05,P<0.01), whereas the apoptosis rate was significantly higher(P<0.01). The level of semen plasma Leptin was higher than that of the control (P<0.05), moreover itwas positively correlated with sperm apoptosis rate (r=0.678,P<0.05). After treatment, semen activity was increased and sperm apoptosis rate and Leptin level were decreased in WBC group. Conclusion Increase inseminal Leptin level and sperm apoptosis rate were founded in WBC group, which may be contributed to the decrease of sperm concentration and viability. Positive relationship between seminal Leptin and sperm apoptosis was found, implyingthat Leptin might play a role in sperm apoptosis by increasing seminal leukocyte.
