CAJ | 학술논문

目的：探讨补肾生精汤对铅损伤小鼠生精细胞凋亡的影响，为临床治疗重金属所致男性不育提供理论依据。方法成年健康清洁级ICR雄性小鼠30只，随机分为对照组、模型组和给药组，每组10只。醋酸铅灌胃建立睾丸损伤模型（30mg/kg体质量，0.1ml/10g容积），采用中药补肾生精汤灌胃治疗（15.0g/kg，即25mL/kg体质量），每天1次，共28d。TUNEL法检测细胞凋亡情况，共聚焦激光扫描显微镜观察；JC-1染色法检测线粒体膜电位变化情况，NucleoCounter NC-3000TM对线粒体膜电位进行分析。结果 TUNEL法检测，模型组凋亡细胞数量（11.60±2.01）明显高于对照组（1.20±1.03）（P ＜0.01），阳性细胞多发生于精原细胞和精母细胞的位置；给药组凋亡细胞数（3.40±1.78）明显低于模型组（P ＜0.01）。JC-1染色法检测，模型组去极化细胞（凋亡细胞）比例为（74.20%±1.3038），明显高于对照组（31.80%±0.8367）（P ＜0.01）；给药组去极化细胞比例为（54.20%±1.3038），低于模型组（P＜0.01）。结论补肾生精汤可通过抑制生精细胞凋亡而改善铅损伤小鼠的生精功能。
목적：탐토보신생정탕대연손상소서생정세포조망적영향，위림상치료중금속소치남성불육제공이론의거。방법성년건강청길급ICR웅성소서30지，수궤분위대조조、모형조화급약조，매조10지。작산연관위건립고환손상모형（30mg/kg체질량，0.1ml/10g용적），채용중약보신생정탕관위치료（15.0g/kg，즉25mL/kg체질량），매천1차，공28d。TUNEL법검측세포조망정황，공취초격광소묘현미경관찰；JC-1염색법검측선립체막전위변화정황，NucleoCounter NC-3000TM대선립체막전위진행분석。결과 TUNEL법검측，모형조조망세포수량（11.60±2.01）명현고우대조조（1.20±1.03）（P ＜0.01），양성세포다발생우정원세포화정모세포적위치；급약조조망세포수（3.40±1.78）명현저우모형조（P ＜0.01）。JC-1염색법검측，모형조거겁화세포（조망세포）비례위（74.20%±1.3038），명현고우대조조（31.80%±0.8367）（P ＜0.01）；급약조거겁화세포비례위（54.20%±1.3038），저우모형조（P＜0.01）。결론보신생정탕가통과억제생정세포조망이개선연손상소서적생정공능。
Objective To investigate the effects of Bu Shen Sheng Jing Tang on apoptosis of spermatogenic cells in lead-treated mice, and establish the theoretical basis for clinical treatment of male infertility induced by heavy metals . Methods Total of 30 adult male ICR mice were randomly divided into three groups such as the control group, the model group and the treated group. Testis-injuried model was made by intragastric administration of lead acetate(30mg?kg-1, 0.1ml?10g-1). Mice in the treated group received intragastric administration of lead acetate and Bu Shen Sheng Jing Tang (15.0g?kg-1, 25mL?kg-1) at the same time for 28days. Mice in the control group received the same amount of 0.9% NaCl by intragastric administration as well. Apoptosis was examined with TUNEL and observed under the confocal laser scanning microscope. Mitochondrial potential was examined with JC-1 and analyzed by NucleoCounter NC-3000TM. Results The number of apoptosis cells(3.40±1.78) in the treated group was lower than that in the model group(11.60±2.01)(P<0.01), moreover the number of apoptosis cells of these two groups were all higher than that of the control group. The rate of depolarization cells(apoptosis cells) (54.20%±1.3038) was lower than that in the model group (74.20%±1.3038) (P<0.01). Conclusion The results suggest that Bu Shen Sheng Jing Tang supplementation protects the testicular spermatogenisis of lead-treated mice by inhibiting spermatogenic cells apoptosis.