CAJ | 학술논문

目的：探讨慢性非细菌性前列腺炎（chronic nonbacterial prostatitis，CNP）疼痛的可能机制。方法自身免疫法建立CNP大鼠模型；Von Frey丝检测机械刺激缩足阈（paw withdrawal threshold，PWT）；进行前列腺病理学检查和免疫组化检测前列腺与脊髓L5-S2节段c-fos、TRPV1的表达变化和相关性。结果造模后大鼠PWT下降，与对照组相比差异有统计学意义（P＜0.05）。前列腺呈现炎症反应，在病变范围、淋巴细胞浸润方面都较对照组显著，差异有统计学意义（P＜0.05）。与对照组相比，模型组前列腺与脊髓背角L5-S2中c-fos、TRPV1阳性表达均上调，差异有统计学意义（P＜0.05）。前列腺中与脊髓中的c-fos表达无相关关系（r =0.027，P =0.454）；并且两者的TRPV1表达也无相关关系（r =0.000，P=0.5）。结论 c-fos、TRPV1可能通过脊髓L5-S2节段的表达上调参与了CNP大鼠疼痛的形成和维持。
목적：탐토만성비세균성전렬선염（chronic nonbacterial prostatitis，CNP）동통적가능궤제。방법자신면역법건립CNP대서모형；Von Frey사검측궤계자격축족역（paw withdrawal threshold，PWT）；진행전렬선병이학검사화면역조화검측전렬선여척수L5-S2절단c-fos、TRPV1적표체변화화상관성。결과조모후대서PWT하강，여대조조상비차이유통계학의의（P＜0.05）。전렬선정현염증반응，재병변범위、림파세포침윤방면도교대조조현저，차이유통계학의의（P＜0.05）。여대조조상비，모형조전렬선여척수배각L5-S2중c-fos、TRPV1양성표체균상조，차이유통계학의의（P＜0.05）。전렬선중여척수중적c-fos표체무상관관계（r =0.027，P =0.454）；병차량자적TRPV1표체야무상관관계（r =0.000，P=0.5）。결론 c-fos、TRPV1가능통과척수L5-S2절단적표체상조삼여료CNP대서동통적형성화유지。
Objective To explore the possible mechanisms of chronic nonbacterial prostatitis pain. Methods The CNP rat model was established by the immune method. The pain threshold were detected by Von Frey filament. The expressions of c-fos and TRPV1 in the prostate and spinal L5- S2 segmental were detected by immunohistochemistry. Results The PWT of rat model group was significantly decreased as compared with that of the control group (P<0.05). There were significant inflammation in prostate tissues of rats. The differences in the scope of lesions and interstitial lymphocytic infiltrates were statistically significant between model group and the control group (P<0.05). The expressions of c-fos and TRPV1 in prostate and spinal cord dorsal horn L5 - S2 in model group were all upregulated remarkably compared with that of the control group (P<0.05). The expression of c-fos and TRPV1 in the prostate had no correlation with that in spinal cord (r =0.027,P =0.454; r = 0.000, P = 0.000). Conclusion Formation of the pain in rats may be associated with upregulation of c-fos and TRPV1 in the spinal L5 - S2 section.