CAJ | 학술논문

目的：研究异常黏液质型阳痿病证大鼠性腺轴形态学的改变。方法选用90只性功能正常的雄性SD大鼠，从中随机抽取10只设为正常对照组，其余80只为造模组，采用芫荽实+菠菜实+湿寒性环境的干预条件建立异常黏液质证候模型。动态观测大鼠生物学表征与勃起功能改变，造模20周后通过APO勃起实验和性行为学实验筛选出阳痿病证模型后，随机分为病证模型组、病证自然反证组和病证药物反证组，反证时间2周，实验终结时，取下丘脑、垂体和睾丸，HE染色，并于光镜和电镜下进行形态学观察。结果（1）下丘脑：正常组组织结构与细胞形态完好。病证模型组基质疏松，提示水肿；超微结构显示神经元核膜界线不清，线粒体水肿，内质网轻微扩张。自然反证组，部分细胞水肿变性；超微结构显示线粒体水肿，粗面内质网有轻度扩张。药物反证组，得到一定程度修复。（2）垂体：正常组结构与细胞形态完好。病证模型组基质疏松，轻度水肿，超微结构可见内质网明显扩张，线粒体普遍空泡样变。自然反证组基质亦疏松，细胞核细胞质边界不清，细胞水肿；超微结构显示大部分线粒体呈空泡样变，粗面内质网扩张。药物反证组基质疏松有所改善，水肿有所减轻。（3）睾丸：正常组结构与细胞形态完好，病证模型组可见生精小管明显变形，生精细胞层数与数量明显减少，间质减少；超微结构显示，生精细胞数量减少且排列紊乱，可见有精原细胞、精母细胞核固缩。自然反证组，生精细胞数量较少，超微结构下可见支持细胞水肿，内质网扩张呈囊泡状。药物反证组生精细胞数量上升，支持细胞增加，管腔内可见一定数量的精子。结论维医异常黏液质阳痿病证大鼠性腺轴可发生明显的病理改变，提示性腺轴的病理性改变可能是该病证发生发展的重要机制之一。目的：研究異常黏液質型暘痿病證大鼠性腺軸形態學的改變。方法選用90隻性功能正常的雄性SD大鼠，從中隨機抽取10隻設為正常對照組，其餘80隻為造模組，採用芫荽實+菠菜實+濕寒性環境的榦預條件建立異常黏液質證候模型。動態觀測大鼠生物學錶徵與勃起功能改變，造模20週後通過APO勃起實驗和性行為學實驗篩選齣暘痿病證模型後，隨機分為病證模型組、病證自然反證組和病證藥物反證組，反證時間2週，實驗終結時，取下丘腦、垂體和睪汍，HE染色，併于光鏡和電鏡下進行形態學觀察。結果（1）下丘腦：正常組組織結構與細胞形態完好。病證模型組基質疏鬆，提示水腫；超微結構顯示神經元覈膜界線不清，線粒體水腫，內質網輕微擴張。自然反證組，部分細胞水腫變性；超微結構顯示線粒體水腫，粗麵內質網有輕度擴張。藥物反證組，得到一定程度脩複。（2）垂體：正常組結構與細胞形態完好。病證模型組基質疏鬆，輕度水腫，超微結構可見內質網明顯擴張，線粒體普遍空泡樣變。自然反證組基質亦疏鬆，細胞覈細胞質邊界不清，細胞水腫；超微結構顯示大部分線粒體呈空泡樣變，粗麵內質網擴張。藥物反證組基質疏鬆有所改善，水腫有所減輕。（3）睪汍：正常組結構與細胞形態完好，病證模型組可見生精小管明顯變形，生精細胞層數與數量明顯減少，間質減少；超微結構顯示，生精細胞數量減少且排列紊亂，可見有精原細胞、精母細胞覈固縮。自然反證組，生精細胞數量較少，超微結構下可見支持細胞水腫，內質網擴張呈囊泡狀。藥物反證組生精細胞數量上升，支持細胞增加，管腔內可見一定數量的精子。結論維醫異常黏液質暘痿病證大鼠性腺軸可髮生明顯的病理改變，提示性腺軸的病理性改變可能是該病證髮生髮展的重要機製之一。
목적：연구이상점액질형양위병증대서성선축형태학적개변。방법선용90지성공능정상적웅성SD대서，종중수궤추취10지설위정상대조조，기여80지위조모조，채용원유실+파채실+습한성배경적간예조건건립이상점액질증후모형。동태관측대서생물학표정여발기공능개변，조모20주후통과APO발기실험화성행위학실험사선출양위병증모형후，수궤분위병증모형조、병증자연반증조화병증약물반증조，반증시간2주，실험종결시，취하구뇌、수체화고환，HE염색，병우광경화전경하진행형태학관찰。결과（1）하구뇌：정상조조직결구여세포형태완호。병증모형조기질소송，제시수종；초미결구현시신경원핵막계선불청，선립체수종，내질망경미확장。자연반증조，부분세포수종변성；초미결구현시선립체수종，조면내질망유경도확장。약물반증조，득도일정정도수복。（2）수체：정상조결구여세포형태완호。병증모형조기질소송，경도수종，초미결구가견내질망명현확장，선립체보편공포양변。자연반증조기질역소송，세포핵세포질변계불청，세포수종；초미결구현시대부분선립체정공포양변，조면내질망확장。약물반증조기질소송유소개선，수종유소감경。（3）고환：정상조결구여세포형태완호，병증모형조가견생정소관명현변형，생정세포층수여수량명현감소，간질감소；초미결구현시，생정세포수량감소차배렬문란，가견유정원세포、정모세포핵고축。자연반증조，생정세포수량교소，초미결구하가견지지세포수종，내질망확장정낭포상。약물반증조생정세포수량상승，지지세포증가，관강내가견일정수량적정자。결론유의이상점액질양위병증대서성선축가발생명현적병리개변，제시성선축적병이성개변가능시해병증발생발전적중요궤제지일。
Objective To study the morphology changes of gonad axis in abmormal balgam syndrome rat model with impotence disease. Methods 90 mature male SD rats were selected in the study, ten of them were randomly chosen as the nomal group;the others were treated with spinach and coriander diet in a cold and humid environment. Rat biological characterization was observed about 20 weeks until the balgam syndrome model was established, then through APO erectile experiment and mating experiment,the rats were randomly divided into impotence model group,spontaneous recovering group and medication group.After 2 weeks counterevidence, the morphology of hypothalamus, pituitary and testis were observed. Results (1) The hypothalamus: The tissue structure and cell morphology of normal group was in good condition. The cell matrix of impotence model group was loose which suggested that there were swelling;ultrastructure showed the boundary of nuclear membrane was not clear, the mitochondria was edema and the rough endoplasmic reticulum was expansion. Spontaneous recovering group, parts of the cells were edema and degeneration, ultrastructure showed showed that there was slight edema in mitochondria, and mild expansion in rough endoplasmic reticulum. Medication group was a little repaired. (2) The pituitary gland: the normal group was good. The impotence model group and spontaneous recovering group, cell matrix was loose, nucleus boundary was not clear, and the cells were swelling; ultrastructure showed that most of the mitochondria was in cavitation sample change, rough endoplasmic reticulum expanded obviously. Medication group ,the matrix loose was improved and the edema was mitigated. (3) The testis: the normal group was good. The impotence model group , seminiferous ducts were obviously deformed, the number of sperm cells was decreased and disordered, some spermatagonial cells and spermatocytes were necrosis. Spontaneous recovering group, the sperm cells was less;ultrastructure showed that the support cells were swelling and endoplasmic reticulum was expanded as vesicle. Medication group,the sperm cells and supporting cells was increased, and there were some sperms in the tube cavity. Conclusion There are significant changes of in gonad axis of abnormal balgam syndrome rat model with impotence disease, which shows that pathological changes of gonad axis may be one of the mechanisms which made this syndrome occurred and developped.