中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2014年
7期
3-7,18
,共6页
薛珂%凡永%刘悦%丁之德
薛珂%凡永%劉悅%丁之德
설가%범영%류열%정지덕
核糖核酸酶T2%雄性生殖系统%精子活动力%小鼠
覈糖覈痠酶T2%雄性生殖繫統%精子活動力%小鼠
핵당핵산매T2%웅성생식계통%정자활동력%소서
ribonuclease T2 (RNase T2)%male reproductive system%sperm motility%mouse
目的：检测核糖核酸酶T2（ribonuclease T2, RNaseT2）在雄性小鼠生殖系统和精子中的表达、定位及其对精子活动力及前向运动的影响。方法运用免疫荧光染色鉴定RNaseT2蛋白在小鼠生殖系统中的表达、定位及其与肌动蛋白（actin）在精子中的共定位关系，通过Real-time PCR检测RNaseT2 mRNA在小鼠生殖系统中的相对表达水平，借助计算机辅助精液分析（CASA）仪检测RNaseT2对小鼠精子的活动力、前向运动的影响。结果（1） RNaseT2蛋白及其mRNA在小鼠附睾头、体、尾组织以及输精管、前列腺及精囊腺分泌液中均有表达，而在睾丸组织中极低表达或未见表达。（2）在小鼠精子上，该蛋白定位于顶体、颈部、尾部鞭毛并与actin的表达定位具有一定的相似性。（3）体外纯化的RNaseT2蛋白可明显抑制小鼠精子的活动力及前向运动，且抑制具有时间及剂量依赖性。结论在雄性生殖系统中，RNaseT2蛋白作为精子运动的调节因子对精子的活动力及前向运动具有明显的抑制作用。
目的：檢測覈糖覈痠酶T2（ribonuclease T2, RNaseT2）在雄性小鼠生殖繫統和精子中的錶達、定位及其對精子活動力及前嚮運動的影響。方法運用免疫熒光染色鑒定RNaseT2蛋白在小鼠生殖繫統中的錶達、定位及其與肌動蛋白（actin）在精子中的共定位關繫，通過Real-time PCR檢測RNaseT2 mRNA在小鼠生殖繫統中的相對錶達水平，藉助計算機輔助精液分析（CASA）儀檢測RNaseT2對小鼠精子的活動力、前嚮運動的影響。結果（1） RNaseT2蛋白及其mRNA在小鼠附睪頭、體、尾組織以及輸精管、前列腺及精囊腺分泌液中均有錶達，而在睪汍組織中極低錶達或未見錶達。（2）在小鼠精子上，該蛋白定位于頂體、頸部、尾部鞭毛併與actin的錶達定位具有一定的相似性。（3）體外純化的RNaseT2蛋白可明顯抑製小鼠精子的活動力及前嚮運動，且抑製具有時間及劑量依賴性。結論在雄性生殖繫統中，RNaseT2蛋白作為精子運動的調節因子對精子的活動力及前嚮運動具有明顯的抑製作用。
목적：검측핵당핵산매T2（ribonuclease T2, RNaseT2）재웅성소서생식계통화정자중적표체、정위급기대정자활동력급전향운동적영향。방법운용면역형광염색감정RNaseT2단백재소서생식계통중적표체、정위급기여기동단백（actin）재정자중적공정위관계，통과Real-time PCR검측RNaseT2 mRNA재소서생식계통중적상대표체수평，차조계산궤보조정액분석（CASA）의검측RNaseT2대소서정자적활동력、전향운동적영향。결과（1） RNaseT2단백급기mRNA재소서부고두、체、미조직이급수정관、전렬선급정낭선분비액중균유표체，이재고환조직중겁저표체혹미견표체。（2）재소서정자상，해단백정위우정체、경부、미부편모병여actin적표체정위구유일정적상사성。（3）체외순화적RNaseT2단백가명현억제소서정자적활동력급전향운동，차억제구유시간급제량의뢰성。결론재웅성생식계통중，RNaseT2단백작위정자운동적조절인자대정자적활동력급전향운동구유명현적억제작용。
Objective To analyze the expression and distribution of RNase T2 in male mouse reproductive system and explore its effects on sperm motility. Methods The expression and localization of RNase T2 in the male mouse reproductive system and its co-localization with actin on mature spermatozoa were detected by indirect immunofluorescence analysis. The expression of RNase T2 in male mouse reproductive system was measured by real-time PCR. The effect of RNase T2 on mouse sperm motility and progressive motility were analyzed by a computer assisted semen analyzer (CASA). Results 1. RNase T2 was expressed in mouse epididymal caput, corpus, and cauda, and secretions of vas deferens, prostate and seminal vesicle, however no Rnase T2 was found in mouse testis. 2. RNase T2 was co-localized with actin on the sperm head, neck and the tail regions. 3. RNase T2 had an inhibitory effect on the sperm motility and progressive motility in a dose-dependent and time-dependent manners. Conclusion As a regulator for sperm motility, RNase T2 in male reproductive system has a definitely inhibitory effect on the motility and progressive motility of mouse spermatozoa.
