CAJ | 학술논문

为了探讨ATP合酶α亚基和腺嘌呤磷酸核糖基转移酶(APRT)与温敏雄性不育系BNS育性的联系，利用荧光实时定量PCR方法，在花药发育的4个重要时期(四分体期、单核期、二核期和三核期)，定量检测ATP合酶α亚基和APRT相关基因在不育及可育条件下花药中的mRNA表达水平。不育条件下， ATP合酶α亚基基因从四分体到二核期表达量持续下降，与可育株相比在单核期表达量显著下降； APRT1在4个时期的表达量低于其在相应可育条件下的表达量，而APRT2基因在 BNS 不育和可育条件下维持较低的表达水平。APRT 相关基因表达量在三核期均有较显著提高，且可育条件下比不育条件下提高更明显。因此认为， ATP合酶α亚基基因与BNS育性转换密切正相关， APRT基因在三核期转录水平的变化与BNS育性转换有一定关系。
위료탐토ATP합매α아기화선표령린산핵당기전이매(APRT)여온민웅성불육계BNS육성적련계，이용형광실시정량PCR방법，재화약발육적4개중요시기(사분체기、단핵기、이핵기화삼핵기)，정량검측ATP합매α아기화APRT상관기인재불육급가육조건하화약중적mRNA표체수평。불육조건하， ATP합매α아기기인종사분체도이핵기표체량지속하강，여가육주상비재단핵기표체량현저하강； APRT1재4개시기적표체량저우기재상응가육조건하적표체량，이APRT2기인재 BNS 불육화가육조건하유지교저적표체수평。APRT 상관기인표체량재삼핵기균유교현저제고，차가육조건하비불육조건하제고경명현。인차인위， ATP합매α아기기인여BNS육성전환밀절정상관， APRT기인재삼핵기전록수평적변화여BNS육성전환유일정관계。
BNS male sterile line is a temperature-sensitive type in wheat. This study aimed at disclosing the role of ATPase αsubmit and the adenine phosphoribosyl transferase (APRT) in BNS male sterility. At four key stages of pollen development, the differential expressions of ATPase α submit gene and APRT were analyzed using fluorescence real-time quantitative PCR (qRT-PCR) technique. Under sterile condition, the relative expression of ATPaseαsubmit gene decreased constantly from tetrad phase to binucleated stage. Particularly, the expression of ATPaseαsubmit gene in uninucleated period was significantly lower in sterile plant than in fertile plant. At four stages, APRT1 had lower expressions under sterile condition than under fertile condition, whereas, APRT2 maintained low expression level in fertility conversion. The transcription levels of APRT genes increased sig-nificantly in trinucleate period, and the variation was more obvious in fertile plants than in sterile plants. Therefore, ATPase αsubmit is positively related to fertility conversion in BNS sterile line and APRT genes probably play a role in trinucleate period.