CAJ | 학술논문

种子硫苷在甘蓝型油菜中有着重要的生物学作用和经济价值。本文旨在通过复合区间作图法利用高密度SNP 遗传连锁图谱定位种子硫苷的 QTL。用近红外扫描获得种子硫苷含量,每株系扫描3次,取平均值。所用的高密度SNP遗传图谱包含2795个SNP多态性标记位点,图谱总长1832.9 cM,相邻标记间平均距离为0.66 cM。定位了2年的种子硫苷含量QTL,其中有5个QTL在2年内被重复检测到,分别分布在A03、A09和C02染色体上, LOD阈值在2.90~10.40之间。这些QTL在2011和2012年试验中分别解释了56.9%和55.1%的表型变异。另外有5个QTL仅在其中一年被检测到,这些QTL能够解释4.1%~7.9%的表型变异, QTL阈值在2.53~3.83之间。
충자류감재감람형유채중유착중요적생물학작용화경제개치。본문지재통과복합구간작도법이용고밀도SNP 유전련쇄도보정위충자류감적 QTL。용근홍외소묘획득충자류감함량,매주계소묘3차,취평균치。소용적고밀도SNP유전도보포함2795개SNP다태성표기위점,도보총장1832.9 cM,상린표기간평균거리위0.66 cM。정위료2년적충자류감함량QTL,기중유5개QTL재2년내피중복검측도,분별분포재A03、A09화C02염색체상, LOD역치재2.90~10.40지간。저사QTL재2011화2012년시험중분별해석료56.9%화55.1%적표형변이。령외유5개QTL부재기중일년피검측도,저사QTL능구해석4.1%~7.9%적표형변이, QTL역치재2.53~3.83지간。
Seed glucosinolate plays important biological and economic roles in Brassica napus. In this study, we aimed at identi-fying QTLs associated with seed glucosinolate content of B. napus using the composite interval mapping (CIM) method based on the high density SNP genetic map. The total seed glucosinolate content was analyzed via near infrared spectroscopy (NIR) using standard methods with three technical replicates. The QTLs associated with seed glucosinolate content were detected using the SNP genetic map constructed in 2013, which contains 2795 SNP markers with the total map length of 1832.9 cM and an average distance of 0.66 cM. Five QTLs for total seed glucosinolate content were identified on linkage groups A03, A09, and C02 in both 2011 and 2012, and their LOD threshold values ranged from 2.90 to 10.40. These QTLs explained 56.9%and 55.1%of pheno-typic variance in 2011 and 2012, respectively. Another five minor QTLs were detected in either 2011 or 2012 with phenotypic contributions between 4.1%and 7.9%, and their LOD threshold values were 2.53-3.83.