CAJ | 학술논문

硬粒小麦籽粒中脂肪氧化酶(LOX)与硬粒小麦面制品的加工品质关系密切相关,而 Lpx-B1位点不同变异类型对LOX活性有重要影响。对来自不同国家和地区的167份硬粒小麦品种的LOX活性进行测定,并对其Lpx-B1位点不同变异类型进行分子鉴定。不同品种间LOX活性差异明显,变幅为0.20~7.98 AU min-1 g-1。在Lpx-B1.1位点鉴定出3种等位变异,分别为Lpx-B1.1a、Lpx-B1.1b和Lpx-B1.1c,以Lpx-B1.1a所占比例最高(55.1%),其次为Lpx-B1.1c (37.1%),而Lpx-B1.1b仅占7.8%；Lpx-B1.2和Lpx-B1.3二者总是互补出现在不同的品种中,146份品种为Lpx-B1.2型,其余21份品种均为Lpx-B1.3型,表明二者可能互为一对等位因子。在Lpx-B1.1位点的3种等位变异类型中, Lpx-B1.1b类型品种的LOX活性显著高于Lpx-B1.1a和Lpx-B1.1c类型品种,而Lpx-B1.1c类型品种的LOX活性最低。Lpx-B1.3类型品种的LOX活性显著高于Lpx-B1.2类型的品种。参试品种共有6种Lpx-B1基因型组合,其中Lpx-B1.1b/Lpx-B1.3基因型的LOX活性显著高于其他基因型,而Lpx-B1.1c/Lpx-B1.2和Lpx-B1.1c/Lpx-B1.3基因型的LOX活性最低。这些观测结果为硬粒小麦品质育种提供了重要信息。
경립소맥자립중지방양화매(LOX)여경립소맥면제품적가공품질관계밀절상관,이 Lpx-B1위점불동변이류형대LOX활성유중요영향。대래자불동국가화지구적167빈경립소맥품충적LOX활성진행측정,병대기Lpx-B1위점불동변이류형진행분자감정。불동품충간LOX활성차이명현,변폭위0.20~7.98 AU min-1 g-1。재Lpx-B1.1위점감정출3충등위변이,분별위Lpx-B1.1a、Lpx-B1.1b화Lpx-B1.1c,이Lpx-B1.1a소점비례최고(55.1%),기차위Lpx-B1.1c (37.1%),이Lpx-B1.1b부점7.8%；Lpx-B1.2화Lpx-B1.3이자총시호보출현재불동적품충중,146빈품충위Lpx-B1.2형,기여21빈품충균위Lpx-B1.3형,표명이자가능호위일대등위인자。재Lpx-B1.1위점적3충등위변이류형중, Lpx-B1.1b류형품충적LOX활성현저고우Lpx-B1.1a화Lpx-B1.1c류형품충,이Lpx-B1.1c류형품충적LOX활성최저。Lpx-B1.3류형품충적LOX활성현저고우Lpx-B1.2류형적품충。삼시품충공유6충Lpx-B1기인형조합,기중Lpx-B1.1b/Lpx-B1.3기인형적LOX활성현저고우기타기인형,이Lpx-B1.1c/Lpx-B1.2화Lpx-B1.1c/Lpx-B1.3기인형적LOX활성최저。저사관측결과위경립소맥품질육충제공료중요신식。
Lipoxygenase (LOX) has a close relationship to the processing quality of flour products in durum wheat (Triticum tur-gidum L.). Activity of LOX is greatly influenced by the locus of Lpx-B1. The Lpx-B1 alleles in 167 durum wheat varieties from different countries or regions were identified using gene-specific primers, and the Lpx-B1 genotypes were analyzed based on LOX activity data. The LOX activity varied greatly in the 167 varieties, ranging from 0.20 to 7.98 AU min-1 g-1. On Lpx-B1.1 locus, three alleles (Lpx-B1.1a, Lpx-B1.1b, and Lpx-B1.1c) were identified with frequencies of 55.1%, 7.8%, and 37.1%, respectively. Loci Lpx-B1.2 and Lpx-B1.3 were always reciprocally present in varieties, in which Lpx-B1.2 was presented in 146 varieties and Lpx-B1.3 in the remaining 21 varieties. This result indicates the allelic possibility of Lpx-B1.2 and Lpx-B1.3. In the three Lpx-B1.1 genotypes, the Lpx-B1.1b varieties had significantly higher LOX activity than the Lpx-B1.1a and Lpx-B1.1c varieties, and varie-ties carrying Lpx-B1.1c allele possessed the lowest LOX activity. The LOX activity in Lpx-B1.3 genotypes was significantly higher than that in Lpx-B1.2 genotypes. A total of six genotypic combinations were found on the three Lpx-B1 loci in 167 varieties. Among them, Lpx-B1.1b/Lpx-B1.3 combination had the highest LOX activity and combinations of Lpx-B1.1c/Lpx-B1.2 and Lpx-B1.1c/Lpx-B1.3 had the lowest LOX activity. These results are informative to quality breeding of durum wheat.