CAJ | 학술논문

目的：建立高效液相色谱法（HPLC）指纹图谱法，测定野山花旗参100目、200目、1000目粉三种粉末中人参皂苷Re、Rb1、Rg1的总含量，通过指纹图谱相似度比较，考察粉碎对野山花旗参中人参皂苷的影响。方法：采用梯度洗脱、HPLC测定三种粒径粉末的HPLC指纹图谱，计算主要药效成分人参皂苷Re、Rb1、Rg1的含量，并进行比较，通过“中药色谱指纹图谱相似度评价系统”评价三个样品的相似度。结果：野山花旗参100目、200目、1000目粉的主要有效成分人参皂苷Re、Rb1、Rg1的总含量均大于3.96%，三种粉末的指纹图谱重叠率高，共有峰的相对保留时间吻合，与生成的对照指纹图谱相似度分别为0.946，0.979，0.970。结论：野山花旗参100目、200目与1000目粉的HPLC指纹图谱相似度大于0.946，化学成分的种类没有变化，主要有效成分人参皂苷Re、Rb1、Rg1的总含量基本相等，野山花旗参超微粉碎成1000目粉，化学成分保持不变。
목적：건립고효액상색보법（HPLC）지문도보법，측정야산화기삼100목、200목、1000목분삼충분말중인삼조감Re、Rb1、Rg1적총함량，통과지문도보상사도비교，고찰분쇄대야산화기삼중인삼조감적영향。방법：채용제도세탈、HPLC측정삼충립경분말적HPLC지문도보，계산주요약효성분인삼조감Re、Rb1、Rg1적함량，병진행비교，통과“중약색보지문도보상사도평개계통”평개삼개양품적상사도。결과：야산화기삼100목、200목、1000목분적주요유효성분인삼조감Re、Rb1、Rg1적총함량균대우3.96%，삼충분말적지문도보중첩솔고，공유봉적상대보류시간문합，여생성적대조지문도보상사도분별위0.946，0.979，0.970。결론：야산화기삼100목、200목여1000목분적HPLC지문도보상사도대우0.946，화학성분적충류몰유변화，주요유효성분인삼조감Re、Rb1、Rg1적총함량기본상등，야산화기삼초미분쇄성1000목분，화학성분보지불변。
Objective:To establish a HPLC fingerprint method for the determination of the total content of Re,Rb1 and Rg1 in ginseng saponins of the wild American ginseng powder(100,200 and 1 000 mesh powder). To investigate the crushing effect on ginseng saponins of wild American ginseng by comparing the similarity of HPLC fingerprints.Methods:Using HPLC and gradient elution,the fingerprints of three kinds of particle size of powders were determined,and the contents of the main effective components of Re,Rb1,Rg1 in ginseng saponin were calculated and compared. Through the Similarity Evaluation System for Chromatographic Fingerprint of TCM,the similarity of the three samples was evaluated. Results:As the main active ingredients of wild American ginseng,the contents of Re,Rb1,Rg1 of ginseng saponin were all greater than 3.96% in the powder of 100,200 and 1 000 mesh,the fingerprint overlap rate of three kinds of powders was high,the relative retention time of the common peak was matched,and the generated fingerprint similarity to the control was 0.946,0.979 and 0.970 respectively.Conclusion:The HPLC fingerprint similarity of wild American ginseng(100,200 and 1 000 mesh powder)was greater than 0.946,the chemical composition of the species was not changed,the contents of Re,Rb1,Rg1 of ginseng saponin as the main active ingredients were generally the same,and when wild American ginsengwas grinded into 1 000 mesh powder,the chemical components remained the same.