中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2014年
8期
821-827
,共7页
林国华%黄明松%程由注%郑瑞丹%颜翠兰%陈韶红%方彦炎%林绍兴%周耀雄
林國華%黃明鬆%程由註%鄭瑞丹%顏翠蘭%陳韶紅%方彥炎%林紹興%週耀雄
림국화%황명송%정유주%정서단%안취란%진소홍%방언염%림소흥%주요웅
医学贝类%种群%生态环境%广州管圆线虫%感染率%检测方法%新宿主
醫學貝類%種群%生態環境%廣州管圓線蟲%感染率%檢測方法%新宿主
의학패류%충군%생태배경%엄주관원선충%감염솔%검측방법%신숙주
medical shellfish%populations%ecological environment%Angiostrongylus cantonensis%infection rate%detec-tion method%new host
目的：调查龙海市医学贝类种群及其广州管圆线虫感染率。方法按不同类型孳生地设调查点，采集水生和陆生贝类。用肺检法检查大瓶螺肺囊，其他贝类用捣碎匀浆法，检查广州管圆线虫Ⅲ期幼虫。比较匀浆法和肺检法在褐云玛瑙螺体组织的检查效果。调查影响贝类感染率的生态环境因素。结果调查9个乡镇27个调查点1673份标本，查出大瓶螺、石环棱螺、铜锈环棱螺、瘤拟黑螺、褐云玛瑙螺、高突足襞蛞蝓、双线嗜粘液蛞蝓和同型巴蜗牛等8种贝类，广州管圆线虫总感染率为19．78％。其中高突足襞蛞蝓最高，达56．63％（47/83），褐云玛瑙螺和大瓶螺分别为39．32％（92/234）与27．14％（130/234）。各调查点感染率高低与其距离居民生活区远近密切相关。首次在瘤拟黑螺内检及广州管圆线虫幼虫。肺检法和匀浆法的检出率分别为87．1％与100．0％，两者差异有统计学意义。结论高突足襞蛞蝓、褐云玛瑙螺和大瓶螺为当地感染广州管圆线虫优势种群，感染率同各种贝类的微生态环境关系密切。瘤拟黑螺充当广州管圆线虫新宿主。肺检法不适合用于褐云玛瑙螺的的广州管圆线虫感染定性筛查。
目的：調查龍海市醫學貝類種群及其廣州管圓線蟲感染率。方法按不同類型孳生地設調查點，採集水生和陸生貝類。用肺檢法檢查大瓶螺肺囊，其他貝類用擣碎勻漿法，檢查廣州管圓線蟲Ⅲ期幼蟲。比較勻漿法和肺檢法在褐雲瑪瑙螺體組織的檢查效果。調查影響貝類感染率的生態環境因素。結果調查9箇鄉鎮27箇調查點1673份標本，查齣大瓶螺、石環稜螺、銅鏽環稜螺、瘤擬黑螺、褐雲瑪瑙螺、高突足襞蛞蝓、雙線嗜粘液蛞蝓和同型巴蝸牛等8種貝類，廣州管圓線蟲總感染率為19．78％。其中高突足襞蛞蝓最高，達56．63％（47/83），褐雲瑪瑙螺和大瓶螺分彆為39．32％（92/234）與27．14％（130/234）。各調查點感染率高低與其距離居民生活區遠近密切相關。首次在瘤擬黑螺內檢及廣州管圓線蟲幼蟲。肺檢法和勻漿法的檢齣率分彆為87．1％與100．0％，兩者差異有統計學意義。結論高突足襞蛞蝓、褐雲瑪瑙螺和大瓶螺為噹地感染廣州管圓線蟲優勢種群，感染率同各種貝類的微生態環境關繫密切。瘤擬黑螺充噹廣州管圓線蟲新宿主。肺檢法不適閤用于褐雲瑪瑙螺的的廣州管圓線蟲感染定性篩查。
목적：조사룡해시의학패류충군급기엄주관원선충감염솔。방법안불동류형자생지설조사점，채집수생화륙생패류。용폐검법검사대병라폐낭，기타패류용도쇄균장법，검사엄주관원선충Ⅲ기유충。비교균장법화폐검법재갈운마노라체조직적검사효과。조사영향패류감염솔적생태배경인소。결과조사9개향진27개조사점1673빈표본，사출대병라、석배릉라、동수배릉라、류의흑라、갈운마노라、고돌족벽활유、쌍선기점액활유화동형파와우등8충패류，엄주관원선충총감염솔위19．78％。기중고돌족벽활유최고，체56．63％（47/83），갈운마노라화대병라분별위39．32％（92/234）여27．14％（130/234）。각조사점감염솔고저여기거리거민생활구원근밀절상관。수차재류의흑라내검급엄주관원선충유충。폐검법화균장법적검출솔분별위87．1％여100．0％，량자차이유통계학의의。결론고돌족벽활유、갈운마노라화대병라위당지감염엄주관원선충우세충군，감염솔동각충패류적미생태배경관계밀절。류의흑라충당엄주관원선충신숙주。폐검법불괄합용우갈운마노라적적엄주관원선충감염정성사사。
The aim of the study was to investigate the population ecology of medical shellfish and the infection of An-giostrongylus cantonensis in Longhai ,Fujian Province ,China .Aquatic and terrestrial shellfish were collected in survey points according to different types of breeding grounds .Then ,lung-microscopy method was involved in the detection of the lung tis-sue in Ampullaria gigas .Other shellfishes were mashed to detect the third-stage larvae of Angiostrongylus cantonensis .Hom-ogenization and lung microscopy were compared in the detection of the larvae of A .cantonensis in Achatina snails .Factors re-lated to the environment and influence of shellfish hosts were also included .Results showed that 8 species of molluscans were found ,including Pila gigas ,Bellamya aeruginosa ,Bellamya lithophaga ,Melanoides tuberculata ,Achatina fulica ,Vag-inulus alte ,Philomycus bilineatus ,and Bradybaenasimilaris with 1 673 specimens in 27 survey points from 9 townships .The infectionratewas19.78% inaverage.TheinfectionrateinV.altewas56.63% (47/83);theinfectionratesforA.fulicaand P .gigas were 39 .32% (92/234) and 27 .14% (130/234) ,respectively .The infection rate of each survey point was closely re-lated to the distances from the residents living area .Morever ,A .cantonensis larvae were detected in M .tuberculata .Lung mi-croscopy and homogenization method detection rate was 87 .1%and 100 .0% ,respectively .The difference was statistically sig-nificant .In conclusion ,V .alte ,A . fulica and P .gigas were A . cantonensist infection dominant population . The infection rate was closely related to micro-ecological environment for all kinds of shellfish .M .tuberculata was the new host of A .can-tonensis .Lung microscopy method should not be used in the qualitative screening detection of A . f ulica infected with A .can-tonensist .
