CAJ | 학술논문

采用高效液相色谱法（HPLC）测定对胍基苯甲酸盐酸盐含量，采用反相C18柱（250 mm ×4.6 mm）；流动相醋酸盐缓冲液（pH=3.0）：乙腈=70：30（V/V），检测波长251 nm，流速0.5 mL/min。结果表明，对胍基苯甲酸盐酸盐在2~30μg/mL范围内线性关系良好，回收率为99.45%。方法准确、简单可行，重复性好，适用于对胍基苯甲酸盐酸盐工业化生产的质量控制。
채용고효액상색보법（HPLC）측정대고기분갑산염산염함량，채용반상C18주（250 mm ×4.6 mm）；류동상작산염완충액（pH=3.0）：을정=70：30（V/V），검측파장251 nm，류속0.5 mL/min。결과표명，대고기분갑산염산염재2~30μg/mL범위내선성관계량호，회수솔위99.45%。방법준학、간단가행，중복성호，괄용우대고기분갑산염산염공업화생산적질량공제。
Using the methods of HPLC,the content of 4-guanidinobenzoic acid hydrochloride is determina-ted. HPLC method used for Kromasil sum C18 column(250 mm × 4. 6 mm);mobile phase:acetate buffer (pH 3. 0): acetonitrile 70:30(V/V),detection wavelength 251 nm,flow rate 0. 5 mL/min. Results showed that the linear ranges of Mecarbinate was 2 ~30 μg/mL,the results of recovery experiment was 99. 45%. Thus the method is accurate,simple,stable and suitable for the quality controlling of Mecarbi-nate during the industrial producing process.