CAJ | 학술논문

以表面共展示具协同催化效应的南极假丝酵母脂肪酶B（ Candida antarctica lipase B，CALB）与嗜热丝孢菌脂肪酶（ Thermomyces lanuginosus lipase，TLL）重组毕赤酵母全细胞作为催化剂催化生物柴油制备，考察了生物柴油制备条件，并进行了初步优化。结果表明，共展示CALB与TLL脂肪酶毕赤酵母全细胞在叔丁醇介质体系中可有效催化生物柴油制备。冻干酵母细胞量0.31 g/g油，醇油摩尔比4：1，温度35℃，反应36 h，生物柴油得率达86.25%。
이표면공전시구협동최화효응적남겁가사효모지방매B（ Candida antarctica lipase B，CALB）여기열사포균지방매（ Thermomyces lanuginosus lipase，TLL）중조필적효모전세포작위최화제최화생물시유제비，고찰료생물시유제비조건，병진행료초보우화。결과표명，공전시CALB여TLL지방매필적효모전세포재숙정순개질체계중가유효최화생물시유제비。동간효모세포량0.31 g/g유，순유마이비4：1，온도35℃，반응36 h，생물시유득솔체86.25%。
Large quantities of Pichia pastoris yeast cells with surface co-displayed Candida antarctica li-pase B( CALB)and Thermomyces lanuginosus lipase( TLL),which former study indicated that the two lipases exhibited significant synergy in catalyzing biodiesel synthesis,were applied to catalyze biodiesel production. The reaction conditions were studied and further optimized. The results indicated that the Pichia pastoris whole-cell catalyst with surface co-displayed CALB and TLL,can effectively catalyzed biodiesel production in t-butanol medium. The optimal reaction conditions for biodiesel production in t-bu-tanol system were:the dried whole-cell loading 0. 31 g/g oil,molar ratio of methanol to oil 4:1,and reac-tion temperature 35 ℃. Under the optimal conditions,reacting for 36 h,the biodiesel yield could reach 86 . 25%.