CAJ | 학술논문

目的：调查分析猪肉屠宰加工过程中弯曲菌的污染现状。方法选择两家猪肉屠宰加工企业，分别采集宰前、脱毛、取内脏、胴体修饰和预冷前后等环节样品，应用选择性 CCDA 平板直接计数法对弯曲菌进行定量检测。结果150份猪肉样品检测出22份弯曲菌阳性样品，阳性率14.67%；环境样品30份，检出12份阳性样品，阳性率40.00%。所检出的弯曲菌均为结肠弯曲菌，未见其他种属弯曲菌。所监测的6个环节均存在不同程度的弯曲菌污染，阳性率呈现先升高后降低的变化趋势。其中取内脏环节弯曲菌阳性率最高，为17.50%，阳性样品弯曲菌携带量为177.14±296.73 CFU/100 cm2；而环境样品中取内脏环节的地面样品阳性率最高，为100%，弯曲菌携带量为588.00±307.66 CFU/100 cm2。结论猪肉屠宰加工过程中存在弯曲菌污染，以取内脏环节阳性率最高，预冷后阳性率明显降低；而环境样品的高污染率对猪肉屠宰加工过程中的交叉污染构成了很大威胁。
목적：조사분석저육도재가공과정중만곡균적오염현상。방법선택량가저육도재가공기업，분별채집재전、탈모、취내장、동체수식화예랭전후등배절양품，응용선택성 CCDA 평판직접계수법대만곡균진행정량검측。결과150빈저육양품검측출22빈만곡균양성양품，양성솔14.67%；배경양품30빈，검출12빈양성양품，양성솔40.00%。소검출적만곡균균위결장만곡균，미견기타충속만곡균。소감측적6개배절균존재불동정도적만곡균오염，양성솔정현선승고후강저적변화추세。기중취내장배절만곡균양성솔최고，위17.50%，양성양품만곡균휴대량위177.14±296.73 CFU/100 cm2；이배경양품중취내장배절적지면양품양성솔최고，위100%，만곡균휴대량위588.00±307.66 CFU/100 cm2。결론저육도재가공과정중존재만곡균오염，이취내장배절양성솔최고，예랭후양성솔명현강저；이배경양품적고오염솔대저육도재가공과정중적교차오염구성료흔대위협。
Objective The research was designed to monitor Campylobacter epidemiology in pork slaughtering and processing links. Methods Two slaughter houses in Jiangsu Province were selected for sampling in live pig, hair removal, evisceration, carcass modification, before and after precooling, using a kind of selective CCDA medium for quantitative detection of Campylobacter. Results The detection rates of campylobacter in 150 carcasses and 30 environmental samples were 14.67% and 40%, respectively. The positive samples are all Campylobacter coli, no other species of Campylobacter were found. In the 6 parts of the monitoring, there are different levels of Campylobacter contamination existed. The highest positive rate is in eviscerating link, which is 17.50%, and the amount of Campylobacter positive samples was 177.14±296.73 CFU/100 cm2. While the highest positive rate of Campylobacter in environmental samples was found in eviscerating links, which was 100%, and the amount of Campylobacter in positive samples was 588±307.66 CFU/100 cm2. Conclusion Campylobacter contaminated pork slaughtering process. Eviscerating link had the highest levels of Campylobacter contamination, but it was reduced obviously after precooling. Furthermore, the high positive rate of environmental samples also indicated that environmental threats were considerable.