食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
8期
2470-2475
,共6页
王国丽%祝洪艳%林海成%匙坤%姚玉莹%张连学
王國麗%祝洪豔%林海成%匙坤%姚玉瑩%張連學
왕국려%축홍염%림해성%시곤%요옥형%장련학
反相高效液相色谱法%五味子醇甲%五味子醇乙%五味子酯甲%五味子甲素%五味子乙素%五味子丙素
反相高效液相色譜法%五味子醇甲%五味子醇乙%五味子酯甲%五味子甲素%五味子乙素%五味子丙素
반상고효액상색보법%오미자순갑%오미자순을%오미자지갑%오미자갑소%오미자을소%오미자병소
reverse phase high performance liquid chromatography%Schisandrol A%Schisandrol B%Schisantherin A%Schizandrin A%Schizandrin B%Schizandrin C
目的：建立反相高效液相色谱法同时测定五味子中6种木脂素含量的方法,并应用此方法检测南、北五味子及3种市售成药：参芪五味子片、安神补心片、安神补心胶囊中此6种木脂素的含量。方法采用SHIMADZU VP-ODS (4.6 mm×150 mm i.d.,5μm)色谱柱,以乙腈-水为流动相,进行梯度洗脱,流速0.5 mL/min,检测波长220 nm,柱温30℃。结果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素分离度良好,分别在0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg的进样量范围内有良好的线性关系,平均加样回收率分别为100.93%、100.59%、99.78%、100.43%、96.72%、102.33%, RSD值分别为2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。结论本研究所建立的反相高效液相色谱法方法准确可靠、简单可行,可用于五味子中木脂素类成分的定量分析,为五味子药材及以五味子为组方药味的中成药的质量评价奠定基础。
目的：建立反相高效液相色譜法同時測定五味子中6種木脂素含量的方法,併應用此方法檢測南、北五味子及3種市售成藥：參芪五味子片、安神補心片、安神補心膠囊中此6種木脂素的含量。方法採用SHIMADZU VP-ODS (4.6 mm×150 mm i.d.,5μm)色譜柱,以乙腈-水為流動相,進行梯度洗脫,流速0.5 mL/min,檢測波長220 nm,柱溫30℃。結果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素分離度良好,分彆在0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg的進樣量範圍內有良好的線性關繫,平均加樣迴收率分彆為100.93%、100.59%、99.78%、100.43%、96.72%、102.33%, RSD值分彆為2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。結論本研究所建立的反相高效液相色譜法方法準確可靠、簡單可行,可用于五味子中木脂素類成分的定量分析,為五味子藥材及以五味子為組方藥味的中成藥的質量評價奠定基礎。
목적：건립반상고효액상색보법동시측정오미자중6충목지소함량적방법,병응용차방법검측남、북오미자급3충시수성약：삼기오미자편、안신보심편、안신보심효낭중차6충목지소적함량。방법채용SHIMADZU VP-ODS (4.6 mm×150 mm i.d.,5μm)색보주,이을정-수위류동상,진행제도세탈,류속0.5 mL/min,검측파장220 nm,주온30℃。결과오미자순갑、오미자순을、오미자지갑、오미자갑소、오미자을소화오미자병소분리도량호,분별재0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg적진양량범위내유량호적선성관계,평균가양회수솔분별위100.93%、100.59%、99.78%、100.43%、96.72%、102.33%, RSD치분별위2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。결론본연구소건립적반상고효액상색보법방법준학가고、간단가행,가용우오미자중목지소류성분적정량분석,위오미자약재급이오미자위조방약미적중성약적질량평개전정기출。
Objective To establish a method for simultaneous determination of six lignans in Schisandra chinensis by reverse phase high performance liquid chromatography (RP-HPLC), and apply this method to detect the content of 6 lignans in Kadsurasinensis, Schisandra chinensis and 3 commercially available patent medicine:Shenqi wuweizi tablets, Anshen buxin tablets, Anshen buxin capsules. Methods The analysis was performed on a SHIMADZU VP-ODS (4.6 mm×150 mm i.d., 5 μm) chromatographic column, and used acetonitrile-water as gradient elution mobile phase, the flow rate was 0.5 mL/min, the detection of wavelength was 220 nm and the column temperature was 30 ℃. Results The method showed an excellent chromatographic separation for the 6 lignans, and showed a good linearity as the injection volume of Schisandrol A, Schisandrol B, Schisantherin A, Schizandrin A, Schizandrin B, and Schizandrin C in 0.243~2.430 μg, 0.087~0.870 μg, 0.060~0.600 μg, 0.093~0.930 μg, 0.246~2.460 μg, and 0.063~0.630 μg. At the same time, the average extraction recoveries were 100.93%, 100.59%, 99.78%, 100.43%, 96.72%, and 102.33%, respectively; the RSD were 2.14% , 2.95%, 2.26%, 2.81% , 1.88% , and 0.985%, respectively. Conclusion The RP-HPLC method established in this study was accurate and reliable, simple and feasible, which could be used to analyze the lignans quantitatively and laid the foundation for the quality evaluation of Schisandra chinensis.
