食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
8期
2457-2461
,共5页
王勇%李蓉%张宪臣%张朋杰%陈定虎%蒋跃明%段学武%李涛涛%胡伟文%杨芳%卢俊文
王勇%李蓉%張憲臣%張朋傑%陳定虎%蔣躍明%段學武%李濤濤%鬍偉文%楊芳%盧俊文
왕용%리용%장헌신%장붕걸%진정호%장약명%단학무%리도도%호위문%양방%로준문
液相色谱串联质谱法%黄曲霉毒素%广式莲蓉月饼%多反应监测%免疫亲和柱
液相色譜串聯質譜法%黃麯黴毒素%廣式蓮蓉月餅%多反應鑑測%免疫親和柱
액상색보천련질보법%황곡매독소%엄식련용월병%다반응감측%면역친화주
liquid chromatography-tandem mass spectrometry%aflatoxins%Cantonese lotus seed paste moon cake%multi-reaction monitor%immunoaffinity column
目的：建立广式莲蓉月饼中的4种黄曲霉毒素(黄曲霉毒素B1、B2、G1、G2)定性和定量的液相色谱-串联质谱检测方法。方法样品经甲醇-水(7：3, v：v)提取后,用免疫亲和柱净化,经甲醇洗脱,在 C18色谱柱上梯度洗脱分离,采用电喷雾正离子多反应监测(MRM)模式测定。结果标准曲线在0.1~20.0 ng/mL范围内线性良好；用本法测定4种黄曲霉毒素的回收率在78.4%~107.8%之间,相对标准偏差在2.5%~9.8%之间。采用该方法对市售5种广式莲蓉月饼中黄曲霉毒素进行分析,其中1份月饼样品能够检测到含有少量的黄曲霉毒素B1(1.2μg/kg)和B2(0.21μg/kg)。结论该方法具有简单快速,准确可靠等特点,适用于广式莲蓉月饼中4种黄曲霉毒素的同时测定。
目的：建立廣式蓮蓉月餅中的4種黃麯黴毒素(黃麯黴毒素B1、B2、G1、G2)定性和定量的液相色譜-串聯質譜檢測方法。方法樣品經甲醇-水(7：3, v：v)提取後,用免疫親和柱淨化,經甲醇洗脫,在 C18色譜柱上梯度洗脫分離,採用電噴霧正離子多反應鑑測(MRM)模式測定。結果標準麯線在0.1~20.0 ng/mL範圍內線性良好；用本法測定4種黃麯黴毒素的迴收率在78.4%~107.8%之間,相對標準偏差在2.5%~9.8%之間。採用該方法對市售5種廣式蓮蓉月餅中黃麯黴毒素進行分析,其中1份月餅樣品能夠檢測到含有少量的黃麯黴毒素B1(1.2μg/kg)和B2(0.21μg/kg)。結論該方法具有簡單快速,準確可靠等特點,適用于廣式蓮蓉月餅中4種黃麯黴毒素的同時測定。
목적：건립엄식련용월병중적4충황곡매독소(황곡매독소B1、B2、G1、G2)정성화정량적액상색보-천련질보검측방법。방법양품경갑순-수(7：3, v：v)제취후,용면역친화주정화,경갑순세탈,재 C18색보주상제도세탈분리,채용전분무정리자다반응감측(MRM)모식측정。결과표준곡선재0.1~20.0 ng/mL범위내선성량호；용본법측정4충황곡매독소적회수솔재78.4%~107.8%지간,상대표준편차재2.5%~9.8%지간。채용해방법대시수5충엄식련용월병중황곡매독소진행분석,기중1빈월병양품능구검측도함유소량적황곡매독소B1(1.2μg/kg)화B2(0.21μg/kg)。결론해방법구유간단쾌속,준학가고등특점,괄용우엄식련용월병중4충황곡매독소적동시측정。
Objective A method for the determination of four aflatoxins in moon paste cake containing lotus seed produced in Canton using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. Methods The sample was extracted with methanol-water (7:3, v:v) solution, followed by a cleanup procedure with immunoaffinity column, and then eluted with 1 mL methanol. Four aflatoxins were separated on a C18 column within 7 min using a mobile phase of acetonitrile and ammonium acetate solution and then detected using electrospray ionization (ESI) MS/MS in positive ion mode with multiple reaction monitoring (MRM). Results The linear ranges of aflatoxin were 0.1~20.0 ng/mL, and the recovery rates for the four aflatoxins were 78.4%~107.8%, while the relative standard deviations were 2.5%~9.8%. Little amounts of aflatoxin B1 (1.2 μg/kg) and B2 (0.21 μg/kg) were detected in one of five moon paste cakes containing lotus seed using this method. Conclusion The developed method is simple, fast, accurate, and it can be applied for the simultaneous determination of four aflatoxins in Cantonese moon paste cake.
