CAJ | 학술논문

目的：全面分析牡丹籽油的脂肪酸组成，为牡丹籽油的保真和掺伪鉴别提供基础。方法采用氢氧化钾甲醇溶液对牡丹籽油中的脂肪酸甲酯化得到脂肪酸甲酯，采用 DB-23毛细管柱对脂肪酸甲酯进行分离，利用质谱选择离子扫描的方式进行检测分析。根据脂肪酸甲酯的质谱特征确定脂肪酸的类型，并识别出样品中的直链饱和脂肪酸甲酯，利用样品中的直链饱和脂肪酸甲酯的保留时间计算其它脂肪酸甲酯的等效链长，然后与相同或相近色谱条件下测定标准品的等效链长比较确定其它脂肪酸甲酯的结构，并利用其质谱特征进行验证。结果本方法可以在不使用标准品的情况下鉴定出牡丹籽油中27种脂肪酸。结论本法测定的牡丹籽油脂肪酸数据明显多于现有方法，为全面认识牡丹籽油的脂肪酸组成并将之应用于保真和掺伪鉴别提供基础。
목적：전면분석모단자유적지방산조성，위모단자유적보진화참위감별제공기출。방법채용경양화갑갑순용액대모단자유중적지방산갑지화득도지방산갑지，채용 DB-23모세관주대지방산갑지진행분리，이용질보선택리자소묘적방식진행검측분석。근거지방산갑지적질보특정학정지방산적류형，병식별출양품중적직련포화지방산갑지，이용양품중적직련포화지방산갑지적보류시간계산기타지방산갑지적등효련장，연후여상동혹상근색보조건하측정표준품적등효련장비교학정기타지방산갑지적결구，병이용기질보특정진행험증。결과본방법가이재불사용표준품적정황하감정출모단자유중27충지방산。결론본법측정적모단자유지방산수거명현다우현유방법，위전면인식모단자유적지방산조성병장지응용우보진화참위감별제공기출。
Objective To fully analyze the fatty acid composition of peony seed oil for subsequent potential adulteration detection. Methods Fatty acids in peony seed oil were derived and analyzed by gas chromatography-mass spectrometry(GC-MS) in selected ion monitoring mode (SIM). Subsequently, structure identification of FAMEs in the peony seed oil was conducted by comparing their mass spectral characteristics and equivalent chain length (ECL) values with those of the fatty acids in Chrombox. The mass spectral characteristics were used to determine the kinds of FAMEs. The ECLs were calculated and used to infer the structure of FAMEs. Finally, fatty acids in peony seed oils were identified and partially validated by reference standards. Results Fatty acids in peony seed oil were identified and partially validated by reference standards. The results showed that 27 fatty acids were identified in peony seed oil. Conclusion The identification method based on the combination of ECL and MS could identify more fatty acids than the existing methods without using reference standards and might benefit the subsequent potential adulteration detection of peony seed oil.