食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
8期
2350-2355
,共6页
海参精囊%环磷酰胺%细胞凋亡%Bcl-2%Bax
海參精囊%環燐酰胺%細胞凋亡%Bcl-2%Bax
해삼정낭%배린선알%세포조망%Bcl-2%Bax
holothurian spermatophore%cyclophosphamide%apoptosis%Bcl-2%Bax
目的:探讨海参精囊水提物对模型小鼠睾丸生精细胞凋亡的影响及相关蛋白的表达情况。方法昆明种小鼠72只随机分为6组,除正常空白组用生理盐水外其他各组均腹腔注射环磷酰胺(CP,28 mg/kg),每天1次,连续5 d,复制睾丸生精障碍小鼠模型;同时治疗组每天灌胃不同剂量的药物1次,连续4周。用TUNEL法检测各组小鼠睾丸生精细胞的凋亡情况;用免疫组化的方法检测Bcl-2和Bax的表达,并与模型组进行比较。结果海参精囊水提物能够抑制小鼠睾丸生精细胞的凋亡,使Bcl-2的表达增加, Bax的表达降低,与模型组比较有统计学意义(P<0.05、0.01)。结论海参精囊水提物对环磷酰胺所致生精障碍模型小鼠的生精细胞凋亡有一定的抑制作用,其作用可能与调控Bcl-2和Bax的表达有关。
目的:探討海參精囊水提物對模型小鼠睪汍生精細胞凋亡的影響及相關蛋白的錶達情況。方法昆明種小鼠72隻隨機分為6組,除正常空白組用生理鹽水外其他各組均腹腔註射環燐酰胺(CP,28 mg/kg),每天1次,連續5 d,複製睪汍生精障礙小鼠模型;同時治療組每天灌胃不同劑量的藥物1次,連續4週。用TUNEL法檢測各組小鼠睪汍生精細胞的凋亡情況;用免疫組化的方法檢測Bcl-2和Bax的錶達,併與模型組進行比較。結果海參精囊水提物能夠抑製小鼠睪汍生精細胞的凋亡,使Bcl-2的錶達增加, Bax的錶達降低,與模型組比較有統計學意義(P<0.05、0.01)。結論海參精囊水提物對環燐酰胺所緻生精障礙模型小鼠的生精細胞凋亡有一定的抑製作用,其作用可能與調控Bcl-2和Bax的錶達有關。
목적:탐토해삼정낭수제물대모형소서고환생정세포조망적영향급상관단백적표체정황。방법곤명충소서72지수궤분위6조,제정상공백조용생리염수외기타각조균복강주사배린선알(CP,28 mg/kg),매천1차,련속5 d,복제고환생정장애소서모형;동시치료조매천관위불동제량적약물1차,련속4주。용TUNEL법검측각조소서고환생정세포적조망정황;용면역조화적방법검측Bcl-2화Bax적표체,병여모형조진행비교。결과해삼정낭수제물능구억제소서고환생정세포적조망,사Bcl-2적표체증가, Bax적표체강저,여모형조비교유통계학의의(P<0.05、0.01)。결론해삼정낭수제물대배린선알소치생정장애모형소서적생정세포조망유일정적억제작용,기작용가능여조공Bcl-2화Bax적표체유관。
Objective To investigate the effects of holothurian spermatophore water extraction on spermatogenic cells apoptosis and Bcl-2, Bax gene expression in model mice. Methods 72 male Kunming mice were equally allotted to 6 groups, and were intraperitoneally injected with cyclophosphamide(CP, 28 mg/kg) once a day for 5 d to construct dyszoospermia model of mice by CP except that the regular group were intraperitoneally injected with normal saline. Mice in the treatment groups orally received holothurian spermatophore water extraction once a day for 4 weeks. Determine the apoptosis of spermatogenic cells by TUNEL. The expression of Bcl-2 and Bax was determined by immunohistochemistry and was compared to those of the model group. Results Holothurian spermatophore water extraction inhibited significantly the apoptosis of testicle spermatogenic cells, up-regulated the expression of Bcl-2, and down-regulated the expression of Bax. The result had a statistical significance(P<0.05, 0.01). Conclusion Holothurian spermatophore water extraction has an inhibitory effect on the apoptosis of spermatogenic cells in mice model of dyszoospermia induced by CP, which may be related to that holothurian spermatophore water extraction can regulate the expression of Bcl-2 and Bax.