CAJ | 학술논문

目的：探讨湿热瘀阻型类风湿关节炎（Rheumatoid arthritis，RA）外周血单个核细胞葡萄糖6-磷酸异构酶（GPI）水平与其他证型类风湿关节炎及正常人群之间的关系。方法采用荧光定量PCR方法检测GPImRNA水平，研究对象包括RA患者47例，其中湿热瘀阻型27例，痰瘀互结型20例，其他风湿病患者对照组20例，30名健康体检者。结果 RA湿热瘀阻组GPImRNA的相对表达量是健康对照组的25．9倍，差异有统计学意义（P＜0．05）。RA痰瘀互结组GPImRNA的相对表达量是健康对照组的6．54倍，而RA湿热瘀阻组与RA痰瘀互结组差异也有统计学意义（P＜0．05）。其他风湿病组GPImRNA的相对表达量是健康对照组的0．97倍，差异无统计学意义（P＞0．05）。结论GPI与RA活动性有关，同时证明湿热瘀阻证是RA活动期的证候之一。
목적：탐토습열어조형류풍습관절염（Rheumatoid arthritis，RA）외주혈단개핵세포포도당6-린산이구매（GPI）수평여기타증형류풍습관절염급정상인군지간적관계。방법채용형광정량PCR방법검측GPImRNA수평，연구대상포괄RA환자47례，기중습열어조형27례，담어호결형20례，기타풍습병환자대조조20례，30명건강체검자。결과 RA습열어조조GPImRNA적상대표체량시건강대조조적25．9배，차이유통계학의의（P＜0．05）。RA담어호결조GPImRNA적상대표체량시건강대조조적6．54배，이RA습열어조조여RA담어호결조차이야유통계학의의（P＜0．05）。기타풍습병조GPImRNA적상대표체량시건강대조조적0．97배，차이무통계학의의（P＞0．05）。결론GPI여RA활동성유관，동시증명습열어조증시RA활동기적증후지일。
Objective To explore the glucose 6 -phosphate isomerase (GPI )level in peripheral blood mononuclear cells in rheumatoid arthritis(RA)of damp-heat-stasis obstruction pattern as well as the relationship between RA differentiated pattern and normal group.Methods The fluorescence quantitative PCR method was used to detect the level of GPI mRNA.The subjects included 47 RA patients,including 27 cases were differentiated as damp-heat-stasis obstruction and 20 cases of interaction of phlegm and stasis. Additionally,20 cases of other rheumatoid diseases were in a control group and 30 cases in a normal group. Results In RA damp-heat-stasis stagnation group,the relevant expression of GPI mRNA was 25 .9 times higher than that in the normal group,indicating the statistical significant difference(P<0.05 ).The relevant expression of GPI mRNA in RA phlegm-stasis interaction group was 6.54 times higher than that in the nor-mal group.The difference was significant between RA damp-heat-stasis obstruction group and RA phlegm-stasis interaction group(P<0.05 ).The expression of GPI mRNA in the rheumatoid disease group was 0.97 times higher than that in the normal group,without significant difference(P>0.05).Conclusion GPI is related to RA activity.It is proved that damp-heat-stasis obstruction syndrome is one of the disorders of RA in active stage.