合肥工业大学学报(自然科学版)
閤肥工業大學學報(自然科學版)
합비공업대학학보(자연과학판)
JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE)
2014年
8期
987-991
,共5页
李建伟%李湘凌%周涛发%袁峰
李建偉%李湘凌%週濤髮%袁峰
리건위%리상릉%주도발%원봉
硝化速率法%半最大效应浓度(EC50)%联合毒性%胞外聚合物(EPS)
硝化速率法%半最大效應濃度(EC50)%聯閤毒性%胞外聚閤物(EPS)
초화속솔법%반최대효응농도(EC50)%연합독성%포외취합물(EPS)
nitrification rate method%concentration for 50% of maximal effect (EC50 )%joint toxicity%extracellular polymeric substance(EPS)
文章采用硝化速率法研究了重金属元素 Pb、Cu的毒性,分析了重金属 Pb、Cu对污泥胞外聚合物(EPS)的影响,初步探讨了重金属毒性机理。研究结果表明:Cu和Pb均是氨氧化细菌的高毒物质,其半最大效应浓度EC50分别是63.60、77.40 mg/L ;Cu/Pb的联合作用方式为协同效应;对细胞活性产生促进、抑制的ρ(Cu)范围分别是0.01~1 mg/L和1~50 mg/L ,ρ(Cu)=100 mg/L是细胞死亡、结构破坏的临界点;ρ(Pb)<1 mg/L可以促进细胞活性,而ρ(Pb)>1 mg/L后,细胞活性降低,且随着ρ(Pb)增加细胞结构破损加剧。
文章採用硝化速率法研究瞭重金屬元素 Pb、Cu的毒性,分析瞭重金屬 Pb、Cu對汙泥胞外聚閤物(EPS)的影響,初步探討瞭重金屬毒性機理。研究結果錶明:Cu和Pb均是氨氧化細菌的高毒物質,其半最大效應濃度EC50分彆是63.60、77.40 mg/L ;Cu/Pb的聯閤作用方式為協同效應;對細胞活性產生促進、抑製的ρ(Cu)範圍分彆是0.01~1 mg/L和1~50 mg/L ,ρ(Cu)=100 mg/L是細胞死亡、結構破壞的臨界點;ρ(Pb)<1 mg/L可以促進細胞活性,而ρ(Pb)>1 mg/L後,細胞活性降低,且隨著ρ(Pb)增加細胞結構破損加劇。
문장채용초화속솔법연구료중금속원소 Pb、Cu적독성,분석료중금속 Pb、Cu대오니포외취합물(EPS)적영향,초보탐토료중금속독성궤리。연구결과표명:Cu화Pb균시안양화세균적고독물질,기반최대효응농도EC50분별시63.60、77.40 mg/L ;Cu/Pb적연합작용방식위협동효응;대세포활성산생촉진、억제적ρ(Cu)범위분별시0.01~1 mg/L화1~50 mg/L ,ρ(Cu)=100 mg/L시세포사망、결구파배적림계점;ρ(Pb)<1 mg/L가이촉진세포활성,이ρ(Pb)>1 mg/L후,세포활성강저,차수착ρ(Pb)증가세포결구파손가극。
The toxicity of heavy metal elements Pb ,Cu was studied by nitrification rate method ,the effect of Pb ,Cu on the extracellular polymeric substances (EPS) was analyzed ,and the toxicity mech-anism of heavy metals was discussed .The research results show that Cu and Pb are high toxic sub-stances to ammonia oxidizing bacteria , the concentration for 50% of maximal effect (EC50 ) is 63.60 mg/L and 77.40 mg/L respectively .T he Cu/Pb joint mode of action is a synergistic effect .T he Cu concentration w hich can promote and inhibit the cell activity is in the range of 0.01-1 mg/L and 1-50 mg/L respectively .T he Cu concentration of 100 mg/L is a critical point of cell death and structural damage .The Pb concentration of less than 1 mg/L can promote the cell activity ,however ,when the Pb concentration is greater than 1 mg/L ,the cell activity is reduced .And the damage to the cell struc-ture exacerbates with the increase of Pb concentration .