化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2014年
8期
75-77
,共3页
红松%多糖%纯化%单糖组成%结构
紅鬆%多糖%純化%單糖組成%結構
홍송%다당%순화%단당조성%결구
Pinus koraiensis%polysaccharide%purification%monosaccharide composition%structure
采用膜分离技术对红松松子壳热水提取物进行分离纯化，经脱蛋白、脱色后得到总多糖 PPSPⅠ-b，通过 DE-AE-52离子交换纤维素柱层析分离得到纯化组分 PPSPⅠ-1，采用气相色谱和红外光谱分析了多糖样品的单糖组成及结构特征。气相色谱分析表明：PPSPⅠ-b 主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成，其物质的量比为3.40∶1.40∶0.79∶7.82∶10.67∶18.35；PPSPⅠ-1主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，其物质的量比为0.96∶2.30∶2.97∶10.60∶6.91。红外光谱分析表明，PPSPⅠ-1具有多糖的特征吸收峰。
採用膜分離技術對紅鬆鬆子殼熱水提取物進行分離純化，經脫蛋白、脫色後得到總多糖 PPSPⅠ-b，通過 DE-AE-52離子交換纖維素柱層析分離得到純化組分 PPSPⅠ-1，採用氣相色譜和紅外光譜分析瞭多糖樣品的單糖組成及結構特徵。氣相色譜分析錶明：PPSPⅠ-b 主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖組成，其物質的量比為3.40∶1.40∶0.79∶7.82∶10.67∶18.35；PPSPⅠ-1主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖組成，其物質的量比為0.96∶2.30∶2.97∶10.60∶6.91。紅外光譜分析錶明，PPSPⅠ-1具有多糖的特徵吸收峰。
채용막분리기술대홍송송자각열수제취물진행분리순화，경탈단백、탈색후득도총다당 PPSPⅠ-b，통과 DE-AE-52리자교환섬유소주층석분리득도순화조분 PPSPⅠ-1，채용기상색보화홍외광보분석료다당양품적단당조성급결구특정。기상색보분석표명：PPSPⅠ-b 주요유서리당、아랍백당、목당、감로당、포도당화반유당조성，기물질적량비위3.40∶1.40∶0.79∶7.82∶10.67∶18.35；PPSPⅠ-1주요유서리당、아랍백당、감로당、포도당화반유당조성，기물질적량비위0.96∶2.30∶2.97∶10.60∶6.91。홍외광보분석표명，PPSPⅠ-1구유다당적특정흡수봉。
The crude polysaccharides were isolated and purified from hot-water extracts of Pinus koraiensis pinon shell by membrane separation method.The total polysaccharides PPSPⅠ-b was obtained by deproteiniza-tion and decolorization,and then the PPSPⅠ-1 fraction was obtained by DEAE-52 cellulose ion-exchange chro-matography.The monosaccharide composition and structure were analyzed by GC and FTIR spectroscopy.GC Analysis showed that PPSPⅠ-b was mainly composed of rhamnose,arabinose,xylose,mannose,glucose,and ga-lactose with the molar ratio of 3.40∶ 1.40∶0.79 ∶ 7.82 ∶ 10.67 ∶ 18.35;PPSPⅠ-1 was mainly composed of rhamnose,arabinose,mannose,glucose,and galactose with the molar ratio of 0.96∶2.30∶2.97∶10.60∶6.91. FTIR Spectrum showed PPSPⅠ-1 had characteristic absorption peaks of polysaccharide.
