CAJ | 학술논문

采用均匀设计法优化了达托霉素发酵培养基配方，并对30 L 罐生产工艺进行了优化。确定达托霉素最优发酵培养基配方为：葡萄糖2.0％、淀粉2.0％、冷榨豆饼粉0.5％、蛋白胨0.6％；以该配方进行30 L 发酵罐生产，当前体癸酸钠的补料起始时间为发酵开始28 h、补料总量为1.5％时，最终发酵效价提高了81％。对达托霉素的工业化生产具有重要的指导意义。
채용균균설계법우화료체탁매소발효배양기배방，병대30 L 관생산공예진행료우화。학정체탁매소최우발효배양기배방위：포도당2.0％、정분2.0％、랭자두병분0.5％、단백동0.6％；이해배방진행30 L 발효관생산，당전체계산납적보료기시시간위발효개시28 h、보료총량위1.5％시，최종발효효개제고료81％。대체탁매소적공업화생산구유중요적지도의의。
Uniform design was used to optimize the medium of daptomycin shaking flask fermentation.The process was also optimized in 30 L fermentor.The optimal fermentation medium was obtained as follows:glu-cose 2.0%,starch 2.0%,soybean flour 0.5%,peptone 0.6%.The fermentation process of daptomycin in 30 L fermentor with above optimum fermentation medium was optimized as follows:the initial feeding time of precur-son sodium caprate was at 28 h,the total feeding amount was 1.5%.The final fermentation potency was im-proved by 81%,which was significant in industrial production of daptomycin.