CAJ | 학술논문

为了探究邻苯二甲酸单丁酯（MBP）对小鼠肝脏和肾脏的氧化损伤，将42只 BALB/c 小鼠随机分为7组，每组6只，分别为25 mg·kg-1、50 mg·kg-1、100 mg·kg-1、200 mg·kg-1的4个 MBP 染毒组、1个100 mg·kg-1的邻苯二甲酸二丁酯（DBP）染毒组、1个空白对照组、1个溶剂对照组。染毒期间对小鼠的体征进行观察；14 d 后取其肝脏和肾脏组织，制作小鼠肝脏和肾脏组织的切片，对肝脏和肾脏的组织学形态进行观察；制作组织匀浆液用于检测肝脏和肾脏组织细胞的 ROS、GSH、MDA 的含量，以了解 MBP 对肝脏和肾脏组织的氧化损伤作用。结果显示：各剂量组小鼠肝细胞和肾小管上皮细胞均出现不同程度的细胞核固缩、细胞水肿、空泡样变、脂肪滴增大融合等症状；ROS 和 MDA 的含量与 MBP 的染毒剂量呈正相关，GSH 的含量与 MBP 的染毒剂量呈负相关；相同剂量的 DBP 与 MBP 染毒组相比，MBP染毒组的 ROS 和 MDA 含量较高、GSH 含量较低。表明，MBP 的暴露与小鼠肝脏和肾脏组织的氧化损伤存在直接联系。
위료탐구린분이갑산단정지（MBP）대소서간장화신장적양화손상，장42지 BALB/c 소서수궤분위7조，매조6지，분별위25 mg·kg-1、50 mg·kg-1、100 mg·kg-1、200 mg·kg-1적4개 MBP 염독조、1개100 mg·kg-1적린분이갑산이정지（DBP）염독조、1개공백대조조、1개용제대조조。염독기간대소서적체정진행관찰；14 d 후취기간장화신장조직，제작소서간장화신장조직적절편，대간장화신장적조직학형태진행관찰；제작조직균장액용우검측간장화신장조직세포적 ROS、GSH、MDA 적함량，이료해 MBP 대간장화신장조직적양화손상작용。결과현시：각제량조소서간세포화신소관상피세포균출현불동정도적세포핵고축、세포수종、공포양변、지방적증대융합등증상；ROS 화 MDA 적함량여 MBP 적염독제량정정상관，GSH 적함량여 MBP 적염독제량정부상관；상동제량적 DBP 여 MBP 염독조상비，MBP염독조적 ROS 화 MDA 함량교고、GSH 함량교저。표명，MBP 적폭로여소서간장화신장조직적양화손상존재직접련계。
To investigate the potential oxidative stress of mono-n-butyl phthalate(MBP)on liver and kidney of mice,42 BALB/c mice were randomly divided into 7 groups,with 6 mice in each group,4 groups were ex-posed to MBP by gavage for 14 d at gradient doses of 25 mg·kg-1 ,50 mg·kg-1 ,100 mg·kg-1 and 200 mg· kg-1 ,one group was exposed to DBP by gavage for 14 d at a dose of 100 mg·kg-1 ,a control group,a vehicle control group.The infected mice signs were observed during the research.The liver and kidney slices were pre-pared to observe the histological changes after 14 d.The contents of ROS,GSH and MDA in the liver and kid-ney homogenate were measured to indicate the oxidative stress.The results showed that,the liver and kidney histological changes were observed in MBP groups,light microscope observation showed hepatocytes and renal tubular cells were karyopyknosis,swelling,ballooned changing and fatty droplets clustering.It also showed that the contents of ROS and MDA increased gradually while GSH content decreased with the increase of exposure dose,and all the studied biomarkers changed in dose-response manners.Under the same concentration,the con-tents of ROS and MDA in MBP exposed groups were all higher than those of DBP infected groups,but the con-tent of GSH decreased.It was demonstrated that MBP could increase the oxidative stress and cause oxidative stress to liver and kidney of mice at relatively high dose.Together,there is a possible relationship between MBP exposure and oxidative stress on liver and kidney of mice.