CAJ | 학술논문

为获得高提取率的灰树花菌丝体多糖，以灰树花菌丝体为原材料，在确定复合酶比例（质量比）及单因素试验的基础上，按L16（215）正交试验设计对超声波协同复合酶法提取灰树花菌丝体多糖的工艺进行优化。结果表明：木瓜蛋白酶∶果胶酶的质量比为2∶1∶2。超声波协同酶法提取灰树花多糖的最佳工艺条件为菌丝干粉与蒸馏水按质量比为100∶1（mL∶g）混合，添加菌粉质量2％的复合酶，调pH为6，超声波处理30 min，在50℃下保温酶解1 h后升温至80℃灭酶，继续恒温浸提1 h，在该条件下，灰树花菌丝体多糖的提取率为39．95％。
위획득고제취솔적회수화균사체다당，이회수화균사체위원재료，재학정복합매비례（질량비）급단인소시험적기출상，안L16（215）정교시험설계대초성파협동복합매법제취회수화균사체다당적공예진행우화。결과표명：목과단백매∶과효매적질량비위2∶1∶2。초성파협동매법제취회수화다당적최가공예조건위균사간분여증류수안질량비위100∶1（mL∶g）혼합，첨가균분질량2％적복합매，조pH위6，초성파처리30 min，재50℃하보온매해1 h후승온지80℃멸매，계속항온침제1 h，재해조건하，회수화균사체다당적제취솔위39．95％。
The technology of ultrasonic + compound enzyme extraction for extracting polysaccharides from G.frondasa mycelium was optimized by L16 (215 )orthogonal design based on single-factor test to obtain high extraction rate of polysaccharides from G.frondasa. The results showed that the polysaccharides extraction rate under the optimum extraction condition of dry mycelium powder 100:distilled water 1 (mL:g ), adding 2% compound enzyme, pH6, ultrasonic treatment for 30min, enzymolysis 1h at 50℃ then enzyme deactivation at 80℃ and finally digestion 1h based on the mass ratio of cellulase 2:papain 1 :pectinase 2 is up to 39.95%.