CAJ | 학술논문

丛枝菌根（arbuscular mycorrhiza，AM）真菌是一类能与高等植物形成菌根的古老共生真菌，由于AM真菌不能纯培养，因此通过传统形态学方法研究存在局限性和不稳定性。近年来，分子生物学方法已被广泛应用于AM真菌多样性的研究。从研究材料选择、DNA的提取、AM真菌特异性引物、分子生物学方法方面综述了分子生物学技术在AM真菌多样性研究中的一般过程以及重要应用，并展望了应用前景。
총지균근（arbuscular mycorrhiza，AM）진균시일류능여고등식물형성균근적고로공생진균，유우AM진균불능순배양，인차통과전통형태학방법연구존재국한성화불은정성。근년래，분자생물학방법이피엄범응용우AM진균다양성적연구。종연구재료선택、DNA적제취、AM진균특이성인물、분자생물학방법방면종술료분자생물학기술재AM진균다양성연구중적일반과정이급중요응용，병전망료응용전경。
Arbuscular mycorrhizal(AM)fungi are ancient obligate symbiotic endophytes,which have a rich diversity in the process of plant co-evolutionary.As AM fungi have not been cultured in vitro,there are significant limitations and instability using morphological methods. Recently, molecular biological methods have been widely used in the study of AM fungal diversity and have made remarkable achievements.The authors outline the process and significant use of molecular biological methods in the study of AM fungal diversity,discuss some related problems,and introduce present and future research trends.