CAJ | 학술논문

为有效地控制贵州兰花的病毒，将经ELISA方法检测为齿兰环斑病毒（ORSV）阳性的蝴蝶兰样品摩擦接种至其枯斑寄主苋色藜进行纯化，RT-PCR 克隆并连接至 pMD18-T 载体后测序，用 DNA-MAN7．0和MEGA5．0软件进行核苷酸序列分析。结果表明：成功克隆该病毒CP基因，其序列长度为477 bp，编码158个氨基酸残基；序列同源性分析显示，该分离物的CP基因与已报道的11种ORSV各地分离物序列同源性高达96％～99％，而与同属其他3种病毒CP基因同源性均低于70％。证明，贵州蝴蝶兰上感染的病毒为 ORSV。
위유효지공제귀주란화적병독，장경ELISA방법검측위치란배반병독（ORSV）양성적호접란양품마찰접충지기고반기주현색려진행순화，RT-PCR 극륭병련접지 pMD18-T 재체후측서，용 DNA-MAN7．0화MEGA5．0연건진행핵감산서렬분석。결과표명：성공극륭해병독CP기인，기서렬장도위477 bp，편마158개안기산잔기；서렬동원성분석현시，해분리물적CP기인여이보도적11충ORSV각지분리물서렬동원성고체96％～99％，이여동속기타3충병독CP기인동원성균저우70％。증명，귀주호접란상감염적병독위 ORSV。
In order to provide a reference for effectively controlling Guizhou orchid virus,a suspected odontoglossum ringspot virus (ORSV)was detected by ELISA and isolated from P.amabilis in Guizhou, and purified by Chenopodium amaranticolor.The coat protein (CP)gene of the isolate was then cloned with RT-PCR,sequenced by pMD18-T vector and analyzed by DNAMAN7.0 and MEGA 5.0 softwares. The results showed that the sequence length of CP gene was 477 bp,and encoded a 158-amino acid CP protein.Result of sequence homogenous analysis showed that the isolate shared 9 6%~9 9% homology with 11 reported ORSV isolates,while less than 70% with other three viruses within the same genus (tobamovirus).Therefore,it could be proposed that the virus isolated from P.amabilis was ORSV.