中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
9期
1289-1293
,共5页
哮喘%Toll样受体2%白细胞介素4%白细胞介素12
哮喘%Toll樣受體2%白細胞介素4%白細胞介素12
효천%Toll양수체2%백세포개소4%백세포개소12
Asthma%Toll-like receptor 2%Interleukin-4%Interleukin-12
目的：观察支气管哮喘（简称哮喘）控制期、未控制期患者及正常对照人群中外周血单个核细胞Toll样受体2（TLR2）mRNA表达及血清白细胞介素4（IL-4）、IL-12水平，并探讨上述指标间有无相关性。方法根据全球哮喘防治创议（GINA）2006哮喘控制水平分级标准，选取门诊急诊哮喘控制期及未控制期患者各20例，选取体检健康者20名作为正常对照组。采用逆转录聚合酶链反应（ RT-PCR）法检测各组对象外周血单个核细胞TLR2 mRNA表达。用酶联免疫吸附测定（ ELISA）双抗体夹心法检测各组对象血清IL-4、IL-12水平。结果哮喘患者外周血单个核细胞 TLR2 mRNA 的表达水平高于正常对照组[（0．963±0．132）比（0．632±0．172）]，差异有统计学意义（P＜0．01）。哮喘控制期与未控制期患者外周血单个核细胞TLR2 mRNA的表达水平差异无统计学意义[（0．936±0．117）比（0．991±0．147）]（ P＞0．05）。哮喘患者外周血IL-4水平高于正常对照组[（34．0±8．2）ng/L比（9．8±2．7）ng/L]，差异有统计学意义（t＝13．87，P＜0．01），未控制组患者外周血IL-4水平高于哮喘控制组[（42．5±5．4）ng/L比（29．4±4．2）ng/L]（t ＝8．53，P＜0．01）。哮喘患者外周血 IL-12水平低于正常对照组[（29．4±3．9）ng/L 比（55．8±6．1）ng/L]（t＝20．54，P＜0．01），哮喘控制组与未控制组患者外周血IL-12水平差异无统计学意义[（28．7±4．5）ng/L比（30．1±3．0）ng/L]（t＝-1．16，P＞0．05）。哮喘患者外周血单个核细胞TLR2 mRNA表达与外周血IL-4水平呈正相关（r＝0．532，P＜0．01），与外周血IL-12无明显相关（r＝-0．05，P＞0．05）。结论哮喘患者外周血单核细胞TLR2 mRNA的表达水平高于正常对照，外周血IL-4水平高于正常对照，未控制期外周血IL-4水平高于控制期，外周血IL-12水平低于正常对照。哮喘患者外周血单核细胞TLR2 mRNA表达与外周血IL-4水平呈正相关，与外周血IL-12无明显相关。
目的：觀察支氣管哮喘（簡稱哮喘）控製期、未控製期患者及正常對照人群中外週血單箇覈細胞Toll樣受體2（TLR2）mRNA錶達及血清白細胞介素4（IL-4）、IL-12水平，併探討上述指標間有無相關性。方法根據全毬哮喘防治創議（GINA）2006哮喘控製水平分級標準，選取門診急診哮喘控製期及未控製期患者各20例，選取體檢健康者20名作為正常對照組。採用逆轉錄聚閤酶鏈反應（ RT-PCR）法檢測各組對象外週血單箇覈細胞TLR2 mRNA錶達。用酶聯免疫吸附測定（ ELISA）雙抗體夾心法檢測各組對象血清IL-4、IL-12水平。結果哮喘患者外週血單箇覈細胞 TLR2 mRNA 的錶達水平高于正常對照組[（0．963±0．132）比（0．632±0．172）]，差異有統計學意義（P＜0．01）。哮喘控製期與未控製期患者外週血單箇覈細胞TLR2 mRNA的錶達水平差異無統計學意義[（0．936±0．117）比（0．991±0．147）]（ P＞0．05）。哮喘患者外週血IL-4水平高于正常對照組[（34．0±8．2）ng/L比（9．8±2．7）ng/L]，差異有統計學意義（t＝13．87，P＜0．01），未控製組患者外週血IL-4水平高于哮喘控製組[（42．5±5．4）ng/L比（29．4±4．2）ng/L]（t ＝8．53，P＜0．01）。哮喘患者外週血 IL-12水平低于正常對照組[（29．4±3．9）ng/L 比（55．8±6．1）ng/L]（t＝20．54，P＜0．01），哮喘控製組與未控製組患者外週血IL-12水平差異無統計學意義[（28．7±4．5）ng/L比（30．1±3．0）ng/L]（t＝-1．16，P＞0．05）。哮喘患者外週血單箇覈細胞TLR2 mRNA錶達與外週血IL-4水平呈正相關（r＝0．532，P＜0．01），與外週血IL-12無明顯相關（r＝-0．05，P＞0．05）。結論哮喘患者外週血單覈細胞TLR2 mRNA的錶達水平高于正常對照，外週血IL-4水平高于正常對照，未控製期外週血IL-4水平高于控製期，外週血IL-12水平低于正常對照。哮喘患者外週血單覈細胞TLR2 mRNA錶達與外週血IL-4水平呈正相關，與外週血IL-12無明顯相關。
목적：관찰지기관효천（간칭효천）공제기、미공제기환자급정상대조인군중외주혈단개핵세포Toll양수체2（TLR2）mRNA표체급혈청백세포개소4（IL-4）、IL-12수평，병탐토상술지표간유무상관성。방법근거전구효천방치창의（GINA）2006효천공제수평분급표준，선취문진급진효천공제기급미공제기환자각20례，선취체검건강자20명작위정상대조조。채용역전록취합매련반응（ RT-PCR）법검측각조대상외주혈단개핵세포TLR2 mRNA표체。용매련면역흡부측정（ ELISA）쌍항체협심법검측각조대상혈청IL-4、IL-12수평。결과효천환자외주혈단개핵세포 TLR2 mRNA 적표체수평고우정상대조조[（0．963±0．132）비（0．632±0．172）]，차이유통계학의의（P＜0．01）。효천공제기여미공제기환자외주혈단개핵세포TLR2 mRNA적표체수평차이무통계학의의[（0．936±0．117）비（0．991±0．147）]（ P＞0．05）。효천환자외주혈IL-4수평고우정상대조조[（34．0±8．2）ng/L비（9．8±2．7）ng/L]，차이유통계학의의（t＝13．87，P＜0．01），미공제조환자외주혈IL-4수평고우효천공제조[（42．5±5．4）ng/L비（29．4±4．2）ng/L]（t ＝8．53，P＜0．01）。효천환자외주혈 IL-12수평저우정상대조조[（29．4±3．9）ng/L 비（55．8±6．1）ng/L]（t＝20．54，P＜0．01），효천공제조여미공제조환자외주혈IL-12수평차이무통계학의의[（28．7±4．5）ng/L비（30．1±3．0）ng/L]（t＝-1．16，P＞0．05）。효천환자외주혈단개핵세포TLR2 mRNA표체여외주혈IL-4수평정정상관（r＝0．532，P＜0．01），여외주혈IL-12무명현상관（r＝-0．05，P＞0．05）。결론효천환자외주혈단핵세포TLR2 mRNA적표체수평고우정상대조，외주혈IL-4수평고우정상대조，미공제기외주혈IL-4수평고우공제기，외주혈IL-12수평저우정상대조。효천환자외주혈단핵세포TLR2 mRNA표체여외주혈IL-4수평정정상관，여외주혈IL-12무명현상관。
Objective To investigate the expression of Toll-like receptor 2(TLR2) mRNA in monocytes of patients′blood during asthma control period , non-control period and healthy people;to investigate serum levels of interleukin-4 (IL-4) and IL-12,and to explore their correlation.Methods According to the standard of GINA 2006 asthma control level , 20 patients were selected for the asthma control period and 20 were selected for the non-control period during emergency and clinic treatment;20 healthy physical examiners were selected for the control group.Expression of TLR2 mRNA was detected by reverse transcription polymerase chain reaction ( RT-PCR) method;serum levels of IL-4 and IL-12 were detected by enzyme linked immunoassay ( ELISA ) .Results Ex-pressions of TLR2 mRNA of the asthma patients was higher than that in normal control group [(0.963 ±0.132) vs (0.632 ±0.172)] (P<0.01).There was no significant difference in the expression of TLR 2 mRNA during asth-ma control period and non-control period[(0.936 ±0.117) vs (0.991 ±0.147)] (P>0.05).Serum level of IL-4 in asthma patients was higher than the normal control group [(34.0 ±8.2)ng/L vs (9.8 ±2.7)ng/L] (t=13.87,P<0.01) with statistically significance .While IL-4 in asthma non-control period patients was higher than that in control period patients[(42.5 ±5.4)ng/L vs (29.4 ±4.2)ng/L] (t=8.53,P<0.01).Serum level of IL-12 of patients was lower than that of normal control group [(29.4 ±3.9)ng/L vs (55.8 ±6.1)ng/L] (t=20.54,P<0.01).While there was no significant statistical difference in the serum level of IL-12 between patients in asthma control period and non-control period [(28.7 ±4.5)ng/L vs (30.1 ±3.0)ng/L] (t=-1.16,P>0.05).Expression of TLR2 mRNA of asthma patients had a positive correlation with serum level of IL-4 ( r=0.532,P<0.01), but there was no obvious correlation to serum level of L-12 (r =-0.05,P >0.05). Conclusions Expression of TLR2 mRNA and IL-4 of the asthma patients is higher than that in the control group patients.Serum level of IL-12 of the patients is lower than the normal control .Expression of TLR2 mRNA of asth-ma patients has a positive correlation with serum level of IL-4, but has no obvious correlation to serum level of IL-12.
