临床医学
臨床醫學
림상의학
CLINICAL MEDICINE
2014年
9期
112-114
,共3页
肺肿瘤%癌前病变%凋亡%半胱氨酸蛋白酶%生存素%原位杂交
肺腫瘤%癌前病變%凋亡%半胱氨痠蛋白酶%生存素%原位雜交
폐종류%암전병변%조망%반광안산단백매%생존소%원위잡교
Lung carcinoma%Precancerous lesion%Apoptosis%Caspase-3%Survivin%Hybridization in situ
目的：研究凋亡效应蛋白Caspase-3和凋亡抑制蛋白Survivin在大鼠肺鳞癌发生发展各阶段的基因转录、表达水平并探讨凋亡和肺癌发生发展的关系。方法选择Wistar大鼠40只，左肺下叶支气管灌注含三甲基胆蒽（3-Methyl-cholanthrene，MCA）和二乙基亚硝胺（ Diethylinitrosamine，DEN）的碘油溶液0.1 ml，另8只灌注0.1 ml碘油做为对照，分批处死获取肺鳞癌形成过程各阶段标本。原位杂交法检测各阶段病变组织中Caspase-3 mRNA和Survivin mRNA的转录水平并采用半定量法进行评分。结果有多个阶段病变共存，共获取支气管黏膜上皮增生18例、不典型增生15例（含鳞状化生7例）、原位癌6例、侵袭癌7例、转移癌6例。正常对照组大鼠支气管黏膜上皮细胞Caspase-3 mRNA表达高于试验组，差异有统计学意义（ P=0.043），其中鳞癌组与正常组相比，差异最显著（ P=0.014）；根据据原位杂交评分，在鳞癌组织中Survivin mRNA表达高于癌前病变组、增生组及正常组（ P﹤0.05）；在鳞癌组中转移癌和非转移组Survivin mR-NA表达评分比较差异有统计学意义（ P﹤0.05）。病变肺组织中，Survivin mRNA和Caspase-3 mRNA呈负相关（ Pearson’s R=-0.647，P﹤0.05）。结论 Caspase-3 mRNA、Survivin mRNA与肺鳞癌的发生和发展相关，Survivin mRNA与Caspase-3 mRNA呈负相关关系，从分子水平验证了Survivin主要通过作用于Caspase-3来阻断细胞的凋亡过程，促进癌变进程。
目的：研究凋亡效應蛋白Caspase-3和凋亡抑製蛋白Survivin在大鼠肺鱗癌髮生髮展各階段的基因轉錄、錶達水平併探討凋亡和肺癌髮生髮展的關繫。方法選擇Wistar大鼠40隻，左肺下葉支氣管灌註含三甲基膽蒽（3-Methyl-cholanthrene，MCA）和二乙基亞硝胺（ Diethylinitrosamine，DEN）的碘油溶液0.1 ml，另8隻灌註0.1 ml碘油做為對照，分批處死穫取肺鱗癌形成過程各階段標本。原位雜交法檢測各階段病變組織中Caspase-3 mRNA和Survivin mRNA的轉錄水平併採用半定量法進行評分。結果有多箇階段病變共存，共穫取支氣管黏膜上皮增生18例、不典型增生15例（含鱗狀化生7例）、原位癌6例、侵襲癌7例、轉移癌6例。正常對照組大鼠支氣管黏膜上皮細胞Caspase-3 mRNA錶達高于試驗組，差異有統計學意義（ P=0.043），其中鱗癌組與正常組相比，差異最顯著（ P=0.014）；根據據原位雜交評分，在鱗癌組織中Survivin mRNA錶達高于癌前病變組、增生組及正常組（ P﹤0.05）；在鱗癌組中轉移癌和非轉移組Survivin mR-NA錶達評分比較差異有統計學意義（ P﹤0.05）。病變肺組織中，Survivin mRNA和Caspase-3 mRNA呈負相關（ Pearson’s R=-0.647，P﹤0.05）。結論 Caspase-3 mRNA、Survivin mRNA與肺鱗癌的髮生和髮展相關，Survivin mRNA與Caspase-3 mRNA呈負相關關繫，從分子水平驗證瞭Survivin主要通過作用于Caspase-3來阻斷細胞的凋亡過程，促進癌變進程。
목적：연구조망효응단백Caspase-3화조망억제단백Survivin재대서폐린암발생발전각계단적기인전록、표체수평병탐토조망화폐암발생발전적관계。방법선택Wistar대서40지，좌폐하협지기관관주함삼갑기담은（3-Methyl-cholanthrene，MCA）화이을기아초알（ Diethylinitrosamine，DEN）적전유용액0.1 ml，령8지관주0.1 ml전유주위대조，분비처사획취폐린암형성과정각계단표본。원위잡교법검측각계단병변조직중Caspase-3 mRNA화Survivin mRNA적전록수평병채용반정량법진행평분。결과유다개계단병변공존，공획취지기관점막상피증생18례、불전형증생15례（함린상화생7례）、원위암6례、침습암7례、전이암6례。정상대조조대서지기관점막상피세포Caspase-3 mRNA표체고우시험조，차이유통계학의의（ P=0.043），기중린암조여정상조상비，차이최현저（ P=0.014）；근거거원위잡교평분，재린암조직중Survivin mRNA표체고우암전병변조、증생조급정상조（ P﹤0.05）；재린암조중전이암화비전이조Survivin mR-NA표체평분비교차이유통계학의의（ P﹤0.05）。병변폐조직중，Survivin mRNA화Caspase-3 mRNA정부상관（ Pearson’s R=-0.647，P﹤0.05）。결론 Caspase-3 mRNA、Survivin mRNA여폐린암적발생화발전상관，Survivin mRNA여Caspase-3 mRNA정부상관관계，종분자수평험증료Survivin주요통과작용우Caspase-3래조단세포적조망과정，촉진암변진정。
Objective To investigate the expression of Caspase-3 and Survivin in the carcinogenesis of lung squamous cell carcinoma in rat. Methods Forty-eight wistar rats were instilled with 3Methylcholanthrene( MCA)and Diethylinitrosamine ( DEN)into the left lobarbranchus to induce lung squamous cell carcinoma,the other 8 rats instilled with iodized oil were regar-ded as control group. The specimens were obtained in every pathological phase durig the carcinogenesis. The expression of Caspase-3 mRNA and Survivin mRNA in each patholonical phases during the carcinogenesis were examined by hybridization in si-tu. The score of hybridization in situ was calculated by combining an estimate of the percentage of immunoreactive cells with that of the stain intensity. Results Sixty specimens with different patholonical phase during the carcinonenesis showed:18 cases of hyperplasia,15 cases of dysplasia( including 7 cases of squamous metaplasia),6 cases of carcinoma in situ,7 cases of invasive camcinoma and 6 cases of metastatic carcinoma. The Caspase-3 mRNA expression levels in control group were higher than those in the test group,there were significant differences(P=0. 043),and the difference in the Caspase-3 mRNA expression levels was the most significant between squamous carcinogenesis group and control group(P=0. 014). The Survivin mRNA expression levels in lung squamous cell carcinoma were higher than those in hyperplasia,precancerous lesions,and control group( P ﹤0. 05). In squamous cell carcinoma group,there was significant difference in the Survivin mRNA expression levels between meta-static carcinoma and non-metastasis carcinoma(P﹤0. 05). In lung squamous cell carcinoma,the expression level of Caspase-3 mRNA was negative correlated with that of Survivin mRNA( Pearson’s R= -0. 647,P﹤0. 05). Conclusion Caspase-3 and Survivin play an important role in the carcinonenesis of rat lung squamous cell carcinoma. The expression of Survivin mRNA is negative correlated with that of Caspase-3 mRNA. Survivin may take part in blocking cell apoptosis by inhibition caspase-3 activi-ty,thereby promote the carcinonenesis of lung cancer.
