CAJ | 학술논문

目的：探讨低分子肝素及肝素结合型表皮生长因子( HB-EGF)对孕早期滋养细胞功能的影响。方法选取体外培养的孕早期绒毛滋养细胞进行研究，采用不同浓度肝素及不同用药搭配进行培养，比较其滋养细胞增殖、侵袭和HCG分泌能力。结果低分子肝素浓度在0.25 U·mL-1和2.5 U·mL-1时则对孕早期滋养细胞增殖和侵袭能力起促进作用(P<0.05)，而在25 U·mL-1及250 U·mL-1时，低分子肝素明显降低滋养细胞侵袭及增殖能力(P<0.05)。联合组对增殖能力和侵袭能力的促进强于另外两组(P<0.05)。低分子肝素组对滋养细胞HCG分泌起抑制作用，而HB-EGF及联合组对HCG分泌具有促进作用(P<0.05)。结论低分子肝素对孕早期滋养细胞的侵袭、增殖以及HCG分泌具有重要作用，而HB-EGF对低分子肝素发挥功能起促进作用。
목적：탐토저분자간소급간소결합형표피생장인자( HB-EGF)대잉조기자양세포공능적영향。방법선취체외배양적잉조기융모자양세포진행연구，채용불동농도간소급불동용약탑배진행배양，비교기자양세포증식、침습화HCG분비능력。결과저분자간소농도재0.25 U·mL-1화2.5 U·mL-1시칙대잉조기자양세포증식화침습능력기촉진작용(P<0.05)，이재25 U·mL-1급250 U·mL-1시，저분자간소명현강저자양세포침습급증식능력(P<0.05)。연합조대증식능력화침습능력적촉진강우령외량조(P<0.05)。저분자간소조대자양세포HCG분비기억제작용，이HB-EGF급연합조대HCG분비구유촉진작용(P<0.05)。결론저분자간소대잉조기자양세포적침습、증식이급HCG분비구유중요작용，이HB-EGF대저분자간소발휘공능기촉진작용。
Objective To investigate the efficacy of modulation of low molecular weight heparin and heparin-binding epidermal growth factor on functions of human first trimester trophoblast. Methods Vitro trimester trophoblast cells were chosen as research objects, which were incubated with different concentrations of heparin and different drugs. And the trophoblast proliferation,invasion and HCG secretion were compared. Results When the concentrations of low molecular weight heparin were 0. 25 U·mL-1 and 2. 5 U·mL-1 , it promoted the first trimester trophoblast cell proliferation and invasion(P<0. 05),while it rose to 25 U·mL-1 and 250 U·mL-1, low molecular weight heparin significantly reduced trophoblast invasion and proliferation(P<0. 05). The combined group was stronger than the other two groups in promoting trophoblast cell invasion and proliferation(P<0. 05). Low molecular weight heparin inhibited trophoblast cells from secreting HCG,while the HB-EGF group and the joint group promoted HCG secretion(P<0. 05). ConclusionsLow molecular weight heparin plays an important role in the first trimester trophoblast invasion,proliferation and secretion,and HB-EGF promotes the role of low molecular weight heparin.