安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2014年
11期
2070-2071,2072
,共3页
高效液相色谱%生发胶囊%二苯乙烯苷
高效液相色譜%生髮膠囊%二苯乙烯苷
고효액상색보%생발효낭%이분을희감
HPLC%Shengfa capsules%2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside
目的：建立生发胶囊中二苯乙烯苷的含量测定方法。方法采用HPLC法,Shim Pack CLC ODS C18柱(150 mm×4.6 mm,5μm),乙腈-水(17：83)为流动相,检测波长320 nm,流速为1.0 mL·min-1,色谱柱温度30℃。结果二苯乙烯苷在5.42~54.2 mg·L-1浓度范围内呈良好的线性关系,r=0.9998(n=6)。平均回收率为97.93%,RSD为1.23%。结论该法准确可靠,可用于生发胶囊的质量控制。
目的：建立生髮膠囊中二苯乙烯苷的含量測定方法。方法採用HPLC法,Shim Pack CLC ODS C18柱(150 mm×4.6 mm,5μm),乙腈-水(17：83)為流動相,檢測波長320 nm,流速為1.0 mL·min-1,色譜柱溫度30℃。結果二苯乙烯苷在5.42~54.2 mg·L-1濃度範圍內呈良好的線性關繫,r=0.9998(n=6)。平均迴收率為97.93%,RSD為1.23%。結論該法準確可靠,可用于生髮膠囊的質量控製。
목적：건립생발효낭중이분을희감적함량측정방법。방법채용HPLC법,Shim Pack CLC ODS C18주(150 mm×4.6 mm,5μm),을정-수(17：83)위류동상,검측파장320 nm,류속위1.0 mL·min-1,색보주온도30℃。결과이분을희감재5.42~54.2 mg·L-1농도범위내정량호적선성관계,r=0.9998(n=6)。평균회수솔위97.93%,RSD위1.23%。결론해법준학가고,가용우생발효낭적질량공제。
Objective To establish a method for the determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in Shengfa cap-sules. Methods HPLC method was used in the quantitative analysis using a Shim Pack CLC ODS C18 column (150 mmí4. 6 mm,5μm). The mobile phase was acetonitrile-water(17:83) and detecting wavelength was 320 nm. The flow rate was 1. 0 mL·min-1 and the column temperature was 30℃. Results The calibration curve had good linear relationship in the range of 5. 42~54. 2 mg· L-1 for 2,3,5,4’-tetrahydroxystilbene 2-O-β-D-glucoside (r =0. 999 8,n =6). The average recovery was 97. 93% and RSD was 1. 23%. Conclusions This method was accurate and reliable,which can be used for the quality control of Shengfa capsules.
