山东工业技术
山東工業技術
산동공업기술
Shandong Industrial Technology
2014年
12期
180-181
,共2页
电化学发光%磁性纳米粒子%电化学发光试
電化學髮光%磁性納米粒子%電化學髮光試
전화학발광%자성납미입자%전화학발광시
Electrochemicaluminescence%Magnetic nanoparticles%ECL reagents
考察了五种不同功能化磁性纳米粒子对Ru(bpy)2+3/TPA体系电化学发光（ECL）强度的影响，并对其中能显著增强ECL强度的磁性纳米粒子的使用条件进行了初步的优化。通过电化学发光检测技术发现BG-Fe、BPG-Fe、PAA-Fe、PE-Fe四种磁性粒子可增强Ru(bpy)32+/TPA体系的ECL强度，增强幅度依次为30%、13%、73%、42%；COOH-MNPs磁性粒子可淬灭该体系的ECL强度，淬灭幅度为23.2%。实验结果表明，浓度为7μg/mL的PAA-Fe磁性纳米粒子经磁铁富集，在电极上富集5min是其促进ECL强度增强的最优条件。本研究对基于功能化磁性纳米粒子开发新的电化学发光试剂和建立新的电化学发光体系具有重要意义。
攷察瞭五種不同功能化磁性納米粒子對Ru(bpy)2+3/TPA體繫電化學髮光（ECL）彊度的影響，併對其中能顯著增彊ECL彊度的磁性納米粒子的使用條件進行瞭初步的優化。通過電化學髮光檢測技術髮現BG-Fe、BPG-Fe、PAA-Fe、PE-Fe四種磁性粒子可增彊Ru(bpy)32+/TPA體繫的ECL彊度，增彊幅度依次為30%、13%、73%、42%；COOH-MNPs磁性粒子可淬滅該體繫的ECL彊度，淬滅幅度為23.2%。實驗結果錶明，濃度為7μg/mL的PAA-Fe磁性納米粒子經磁鐵富集，在電極上富集5min是其促進ECL彊度增彊的最優條件。本研究對基于功能化磁性納米粒子開髮新的電化學髮光試劑和建立新的電化學髮光體繫具有重要意義。
고찰료오충불동공능화자성납미입자대Ru(bpy)2+3/TPA체계전화학발광（ECL）강도적영향，병대기중능현저증강ECL강도적자성납미입자적사용조건진행료초보적우화。통과전화학발광검측기술발현BG-Fe、BPG-Fe、PAA-Fe、PE-Fe사충자성입자가증강Ru(bpy)32+/TPA체계적ECL강도，증강폭도의차위30%、13%、73%、42%；COOH-MNPs자성입자가쉬멸해체계적ECL강도，쉬멸폭도위23.2%。실험결과표명，농도위7μg/mL적PAA-Fe자성납미입자경자철부집，재전겁상부집5min시기촉진ECL강도증강적최우조건。본연구대기우공능화자성납미입자개발신적전화학발광시제화건립신적전화학발광체계구유중요의의。
Herein, the effect of ifve different magnetic nanoparticles (MNPs) on electrochemiluminesence (ECL) intensity of the Ru(bpy)2+3/TPA system were conducted. preliminarily operating conditions of the ideal MNPs were optimized.The result indicated that BG-Fe, BPG-Fe, PAA-Fe and PE-Fe could enhance ECL intensity in the Ru(bpy)2+3/TPA system by method of ECL detection, and the enhanced amplitude of ECL intensity was 30%, 13%, 73% and 42%, respectively. COOH-MNPs could quench the ECL intensity and the amplitude was 23.2%. The utmost ECL enhancement of Ru(bpy)2+3/TPA system was obtained in the optimum conditions as folows: PAA-Fe concentration of was at 7μg/ml and accumulation for 5 minutes by the magnet. The study is of important signiifcance for development of new ECL reagents and establishment of ECL system based on MNPs.
