中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
16期
2993-2996
,共4页
脊髓损伤%细胞凋亡%低睾酮血症%Leydig细胞
脊髓損傷%細胞凋亡%低睪酮血癥%Leydig細胞
척수손상%세포조망%저고동혈증%Leydig세포
Spinal cord injuries%Apoptosis%Low serum testosterone%Leydig cell
目的：探讨脊髓损伤诱发低睾酮血症的机制。方法成年健康SD大鼠20只，随机分为假手术组和脊髓损伤组，手术后14 d取血清以放免法检测睾酮（T）和黄体生成素（LH）水平，以脱氧核糖核酸末端转移酶介导的dUTP的缺口末端标记技术（TUNEL）原位检测Leydig细胞凋亡，以Percoll连续密度梯度法提取Leydig细胞后采用流式细胞仪（FACS）定量检测Leydig细胞凋亡情况。结果术后14 d，脊髓损伤组大鼠血清T水平较假手术组显著降低（P＜0.05），LH水平较假手术组显著升高（P＜0.05），TUNEL显示睾丸间质中Leydig细胞发生凋亡，脊髓损伤组凋亡Leydig细胞比例（46%）显著高于假手术组（17.67%）（P＜0.05）。结论诱发Leydig细胞凋亡可能是脊髓损伤导致低睾酮血症发生的重要机制之一。
目的：探討脊髓損傷誘髮低睪酮血癥的機製。方法成年健康SD大鼠20隻，隨機分為假手術組和脊髓損傷組，手術後14 d取血清以放免法檢測睪酮（T）和黃體生成素（LH）水平，以脫氧覈糖覈痠末耑轉移酶介導的dUTP的缺口末耑標記技術（TUNEL）原位檢測Leydig細胞凋亡，以Percoll連續密度梯度法提取Leydig細胞後採用流式細胞儀（FACS）定量檢測Leydig細胞凋亡情況。結果術後14 d，脊髓損傷組大鼠血清T水平較假手術組顯著降低（P＜0.05），LH水平較假手術組顯著升高（P＜0.05），TUNEL顯示睪汍間質中Leydig細胞髮生凋亡，脊髓損傷組凋亡Leydig細胞比例（46%）顯著高于假手術組（17.67%）（P＜0.05）。結論誘髮Leydig細胞凋亡可能是脊髓損傷導緻低睪酮血癥髮生的重要機製之一。
목적：탐토척수손상유발저고동혈증적궤제。방법성년건강SD대서20지，수궤분위가수술조화척수손상조，수술후14 d취혈청이방면법검측고동（T）화황체생성소（LH）수평，이탈양핵당핵산말단전이매개도적dUTP적결구말단표기기술（TUNEL）원위검측Leydig세포조망，이Percoll련속밀도제도법제취Leydig세포후채용류식세포의（FACS）정량검측Leydig세포조망정황。결과술후14 d，척수손상조대서혈청T수평교가수술조현저강저（P＜0.05），LH수평교가수술조현저승고（P＜0.05），TUNEL현시고환간질중Leydig세포발생조망，척수손상조조망Leydig세포비례（46%）현저고우가수술조（17.67%）（P＜0.05）。결론유발Leydig세포조망가능시척수손상도치저고동혈증발생적중요궤제지일。
Objective To investigate the mechanism involved in low serum testosterone after spinal cord injury.Methods20 adult healthy SD rats were randomly divided into sham-operation group and spinal cord injury (SCI) group. The animals were killed on 14 days after operation. Radioimmunoassay method (RIA) was used to detect serum testosterone and LH level. TUNEL was applied for in vivo detection of apoptotic Leydig cells. Apoptosis of Leydig cells was quantitatively assessed by fluorescence activated cell sorter (FACS).Results On day 14 after operation, serum testosterone levels of SCI group were dramatically lower than sham operation group (P<0.05), but its serum LH levels were significantly higher than that of the latter (P<0.05). By TUNEL, apoptotic Leydig cells were found in the interstitial tissue of testis. The incidence of leydig cell apoptosis in SCI group was 46%, significantly higher than that of sham-operation group (17.67%). Conclusion Leydig cells undergoing apoptosis are one of the underlying mechanisms for serum low testosterone induced by spinal cord injury.
