中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2014年
23期
1-3
,共3页
子痫前期%胎盘%HIF-1α%IL-6
子癇前期%胎盤%HIF-1α%IL-6
자간전기%태반%HIF-1α%IL-6
Preeclampsia%Placenta%HIF-1α%IL-6
目的：探讨缺氧抑制因子-1α(HIF-1α)和白细胞介素-6(IL-6)对子痫前期的发病机制及影响胎儿发育的作用。方法：其中足月择期行剖宫产的健康孕妇40例为正常对照组、子痫前期且胎儿发育正常的孕妇40例作为PE组、子痫前期且胎儿生长受限(FGR)的孕妇40例作为PE+FGR组。运用实时定量PCR(realtime-PCR)技术及蛋白印迹法(WB)检测各组孕妇胎盘组织中HIF-1α和IL-6 mRNA和蛋白的表达。结果：HIF-1α和IL-6 mRNA和蛋白的表达量：PE组和PE+FGR组与正常对照组比较，差异有统计学意义(P<0.01)。但PE组和PE+FGR组中HIF-1α和IL-6 mRNA及蛋白的表达比较，差异有统计学意义(P<0.05)。结论：胎盘性HIF-1α和IL-6对于导致子痫前期的发病及胎儿的正常发育具有重要的作用，为子痫前期的诊断及治疗提供了相关的应对策略。
目的：探討缺氧抑製因子-1α(HIF-1α)和白細胞介素-6(IL-6)對子癇前期的髮病機製及影響胎兒髮育的作用。方法：其中足月擇期行剖宮產的健康孕婦40例為正常對照組、子癇前期且胎兒髮育正常的孕婦40例作為PE組、子癇前期且胎兒生長受限(FGR)的孕婦40例作為PE+FGR組。運用實時定量PCR(realtime-PCR)技術及蛋白印跡法(WB)檢測各組孕婦胎盤組織中HIF-1α和IL-6 mRNA和蛋白的錶達。結果：HIF-1α和IL-6 mRNA和蛋白的錶達量：PE組和PE+FGR組與正常對照組比較，差異有統計學意義(P<0.01)。但PE組和PE+FGR組中HIF-1α和IL-6 mRNA及蛋白的錶達比較，差異有統計學意義(P<0.05)。結論：胎盤性HIF-1α和IL-6對于導緻子癇前期的髮病及胎兒的正常髮育具有重要的作用，為子癇前期的診斷及治療提供瞭相關的應對策略。
목적：탐토결양억제인자-1α(HIF-1α)화백세포개소-6(IL-6)대자간전기적발병궤제급영향태인발육적작용。방법：기중족월택기행부궁산적건강잉부40례위정상대조조、자간전기차태인발육정상적잉부40례작위PE조、자간전기차태인생장수한(FGR)적잉부40례작위PE+FGR조。운용실시정량PCR(realtime-PCR)기술급단백인적법(WB)검측각조잉부태반조직중HIF-1α화IL-6 mRNA화단백적표체。결과：HIF-1α화IL-6 mRNA화단백적표체량：PE조화PE+FGR조여정상대조조비교，차이유통계학의의(P<0.01)。단PE조화PE+FGR조중HIF-1α화IL-6 mRNA급단백적표체비교，차이유통계학의의(P<0.05)。결론：태반성HIF-1α화IL-6대우도치자간전기적발병급태인적정상발육구유중요적작용，위자간전기적진단급치료제공료상관적응대책략。
Objective:To study the oxygen inhibition factor HIF-1αand IL-6 in the pathogenesis of preeclampsia and affect fetal development. Method:Including full line elective cesarean delivery of healthy pregnant women 40 cases as normal control group,preeclampsia,40 cases of normal pregnant women and fetal development as PE group,pre-eclampsia and fetal growth restriction(FGR) 40 cases of pregnant women as PE+FGR group.Using real-time quantitative PCR(realtime-PCR) technology and protein imprinting method(WB) groups of pregnant women in the placenta tissue HIF-1α and IL-6 mRNA and protein expression.Result:HIF-1αand IL-6 mRNA and protein expression:PE and PE+FGR group compared with normal control group,and extremely significant difference was statistically significant(P<0.01).But PE and PE+FGR group of HIF-1α and IL-6 mRNA and protein expression,in contrast,a significant difference,with statistical significance(P<0.05).Conclusion:The placental HIF-1αand IL-6 for leading to the onset of preeclampsia and normal development of the fetus plays an important role,for the diagnosis and treatment of preeclampsia provides relevant countermeasure.
