中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
24期
28-30
,共3页
幽门螺杆菌%免疫印迹法%分型%CagA%VacA
幽門螺桿菌%免疫印跡法%分型%CagA%VacA
유문라간균%면역인적법%분형%CagA%VacA
Helicobacter pylori%Western blot%Serotype%CagA%VacA
目的：用免疫印迹法检测幽门螺杆菌（Hp）感染中不同血清分型与慢性浅表性胃炎（CSG）、慢性萎缩性胃炎（CAG）、消化性溃疡（PU）和胃癌（GC）的关系。方法：选取确诊为Hp感染的上消化道疾病患者205例，用Western blot法（免疫印迹法）测血清幽门螺杆菌抗体谱，检测Hp抗体分型，分析CagA和/或VacA抗体阳性的表达情况。结果：在Hp现症感染中，CSG、CAG、PU、GC患者的HpⅠ型感染比例明显高于Ⅱ型感染（P<0.01）；PU及GC中HpⅠ型感染率高于CSG（P<0.05）。不同年龄段及不同性别中HpⅠ型感染比例均高于Ⅱ型感染（P<0.01）。各组的Hp I型感染者中以CagA+VacA抗体为主，检出率为77.4%；其中以CSG组最低（70.4%）、GC组最高（81.8%）。各组CagA+VacA抗体检出率显著高于单一CagA/VacA抗体检出率（P<0.01）。结论：应用免疫印迹法进行幽门螺杆菌血清学分型检测对于上消化道疾病的判断有临床指导意义，Ⅰ型Hp感染更需要进行根治。
目的：用免疫印跡法檢測幽門螺桿菌（Hp）感染中不同血清分型與慢性淺錶性胃炎（CSG）、慢性萎縮性胃炎（CAG）、消化性潰瘍（PU）和胃癌（GC）的關繫。方法：選取確診為Hp感染的上消化道疾病患者205例，用Western blot法（免疫印跡法）測血清幽門螺桿菌抗體譜，檢測Hp抗體分型，分析CagA和/或VacA抗體暘性的錶達情況。結果：在Hp現癥感染中，CSG、CAG、PU、GC患者的HpⅠ型感染比例明顯高于Ⅱ型感染（P<0.01）；PU及GC中HpⅠ型感染率高于CSG（P<0.05）。不同年齡段及不同性彆中HpⅠ型感染比例均高于Ⅱ型感染（P<0.01）。各組的Hp I型感染者中以CagA+VacA抗體為主，檢齣率為77.4%；其中以CSG組最低（70.4%）、GC組最高（81.8%）。各組CagA+VacA抗體檢齣率顯著高于單一CagA/VacA抗體檢齣率（P<0.01）。結論：應用免疫印跡法進行幽門螺桿菌血清學分型檢測對于上消化道疾病的判斷有臨床指導意義，Ⅰ型Hp感染更需要進行根治。
목적：용면역인적법검측유문라간균（Hp）감염중불동혈청분형여만성천표성위염（CSG）、만성위축성위염（CAG）、소화성궤양（PU）화위암（GC）적관계。방법：선취학진위Hp감염적상소화도질병환자205례，용Western blot법（면역인적법）측혈청유문라간균항체보，검측Hp항체분형，분석CagA화/혹VacA항체양성적표체정황。결과：재Hp현증감염중，CSG、CAG、PU、GC환자적HpⅠ형감염비례명현고우Ⅱ형감염（P<0.01）；PU급GC중HpⅠ형감염솔고우CSG（P<0.05）。불동년령단급불동성별중HpⅠ형감염비례균고우Ⅱ형감염（P<0.01）。각조적Hp I형감염자중이CagA+VacA항체위주，검출솔위77.4%；기중이CSG조최저（70.4%）、GC조최고（81.8%）。각조CagA+VacA항체검출솔현저고우단일CagA/VacA항체검출솔（P<0.01）。결론：응용면역인적법진행유문라간균혈청학분형검측대우상소화도질병적판단유림상지도의의，Ⅰ형Hp감염경수요진행근치。
Objective:To detect the relationship between the different serotype of Helic-obacter pylori infection among the disease of the chronic superficial gastritis,chronic atrophic gastritis,peptic ulcer and gastric cancer using the Western blot.Method:205 patients with upper gastrointestinal diseases who diagnosed Hp infection were selected, the helicobacter pylori antibody spectrum was detected by the Western blot,the positive expression of the CagA and/or VacA antibody were analyzed.Result:Among the patients with Hp infection,the infection ratio of HpⅠamong the disease of CSG,CAG,PU,GC were obviously higher than Hp Ⅱ(P<0.01);Hp Ⅰinfection ratio in PU,GC were higher than in CSG(P<0.05). HpⅠtype infection in the different age groups and different gender ratio were higher than typeⅡinfection(P<0.01). The CagA+VacA antibodies was priority in the HpI type of each groups,the detection rate was 77.4%;the CSG group was the lowest(70.4%),the GC group was the highest(81.8%). CagA+VacA antibody detection rate of each groups was significantly higher than that of single CagA/VacA antibody detection rate(P<0.01). Conclusion:It is significance in clinical to use the Western blot to detect the different serotype of Helicobacter pylori in diagnoses the disease of upper digestive tract. HPⅠinfection should be cured for more reasons.
