CAJ | 학술논문

目的：利用层层自组装技术,在钛表面构建 RGD肽(Arg-Gly-Asp Peptides)多层膜,评价其血液相容性,探讨该技术在医用金属表面改性领域内的应用前景。方法采用氢氧化钠(NaOH)预先处理钛试件,获得多孔、负电荷的钛表面,吸附一层正电荷的聚乙烯亚胺,然后多次交替吸附 RGD肽,形成 RGD肽的多层膜结构。通过对该涂层进行物理表征测定及体外血液相容性检测,评价其血液相容性。结果 RGD肽自组装多层膜表面水接触角为78.21±1.98°,体外溶血率为0.76%,该涂层还可有效延长部分活化凝血酶原时间(APTT)至(15.1±0.2)s,并且能够有效抑制血小板的粘附与激活。结论利用层层自组装技术形成的 RGD肽涂层血液相容性高,有望成为一种新型的生物化钛表面,显示了其在医用金属表面改性领域的广阔应用前景。
목적：이용층층자조장기술,재태표면구건 RGD태(Arg-Gly-Asp Peptides)다층막,평개기혈액상용성,탐토해기술재의용금속표면개성영역내적응용전경。방법채용경양화납(NaOH)예선처리태시건,획득다공、부전하적태표면,흡부일층정전하적취을희아알,연후다차교체흡부 RGD태,형성 RGD태적다층막결구。통과대해도층진행물리표정측정급체외혈액상용성검측,평개기혈액상용성。결과 RGD태자조장다층막표면수접촉각위78.21±1.98°,체외용혈솔위0.76%,해도층환가유효연장부분활화응혈매원시간(APTT)지(15.1±0.2)s,병차능구유효억제혈소판적점부여격활。결론이용층층자조장기술형성적 RGD태도층혈액상용성고,유망성위일충신형적생물화태표면,현시료기재의용금속표면개성영역적엄활응용전경。
Objective To establish RGD peptide multilayers on the surface of titanium by layer-by-layer(LBL)self-assem-bling technique,and to explore the blood compatibility of this titanium and its application in the area of medical metal surface modification.Methods The porous and negatively charged surface of titanium was obtained by pretreatment of titanium with NaOH,which absorbed one layer of positive charged PEI and then alternately RGD peptide to form the multilayer structure of RGD peptide.The blood compatibility of the pure titanium with RGD peptide multilayers was examined in terms of the physical characteristics and in vitro blood compatibility.Results The surface water contact angle of RGD peptide self-assembly multi-layer was (78.21±1.98)°,and the hemolysis rate was 0.76%.The RGD peptide self-assembly multilayer could prolong APTT to (15.1±0.2)s and effectively inhibit platelet adhesion and activation.Conclusion The blood compatibility of pure titanium can be significantly improved after being coated with RGD peptide multilayers,which are expected to become a novel biological tinanium surface and show broad application prospects in the area of medical metal surface modification.