CAJ | 학술논문

目的：检测淋巴管内皮细胞透明质酸受体1(LYVE-1)和同源盒基因转录因子1(PROX-1)在人乳腺癌组织中的表达，探讨淋巴管密度在肿瘤淋巴结转移中的临床意义。方法收集80例乳腺浸润性导管癌和35例乳腺良性增生组织，采用免疫组织化学 SP法检测LYVE-1和PROX-1的表达。结果乳腺癌组织中LYVE-1和PROX-1各自标记的淋巴管密度均明显高于乳腺增生组织，差异均有统计学意义(均P<0.01)，且与肿瘤淋巴结转移密切相关，有淋巴结转移者均高于无淋巴结转移者(均P<0.01)；PROX-1在癌细胞内的表达，不同的肿瘤病理学分级和临床分期之间差异均有统计学意义(均P<0.01)。结论乳腺浸润性导管癌组织中淋巴管生成增多，淋巴管密度与肿瘤淋巴结转移相关， PROX-1可能与乳腺癌发生、发展及淋巴结转移相关。
목적：검측림파관내피세포투명질산수체1(LYVE-1)화동원합기인전록인자1(PROX-1)재인유선암조직중적표체，탐토림파관밀도재종류림파결전이중적림상의의。방법수집80례유선침윤성도관암화35례유선량성증생조직，채용면역조직화학 SP법검측LYVE-1화PROX-1적표체。결과유선암조직중LYVE-1화PROX-1각자표기적림파관밀도균명현고우유선증생조직，차이균유통계학의의(균P<0.01)，차여종류림파결전이밀절상관，유림파결전이자균고우무림파결전이자(균P<0.01)；PROX-1재암세포내적표체，불동적종류병이학분급화림상분기지간차이균유통계학의의(균P<0.01)。결론유선침윤성도관암조직중림파관생성증다，림파관밀도여종류림파결전이상관， PROX-1가능여유선암발생、발전급림파결전이상관。
Objective To investigate the expressions of LYVE-1 and PROX-1 in human breast carcinoma and the clinical significance of lymphatic vessel density.Methods Immunohistochemistry (SP method)was used to detect the expressions of LYVE-1 and PROX-1 in 80 specimens of breast invasive ductal carcinoma and 35 specimens of breast hyperplasia.Results The density of lymphatic vessels positive for LYVE-1 or PROX-1 was significantly higher in breast carcinoma than in breast hyper-plasia (P<0.01).There was a significant correlation between lymphatic vessel density and lymph node metastasis (P<0.01). A negative correlation was noted between the PROX-1 expression in carcinoma cells and tumor grade (P<0.01)or TNM stage (P < 0.01 ).Conclusion Lymphangiogenesis is increased in breast carcinoma,which is associated with lymph node metastasis.PROX-1 may be involved in tumorigenesis,progression and lymphatic metastasis in breast cancer.