CAJ | 학술논문

从8株红曲霉中筛选出红曲霉MQ7作为高产糖化酶和酸性蛋白酶的供试菌株，采用固态发酵方法测定在不同发酵温度、底物料液比、大米和麸皮比例条件下的产酶情况．利用正交实验优化固态发酵产酶过程，确定该菌株产酶最佳发酵条件为：发酵温度30,℃，底物料液比(g∶mL)20∶25，大米麸皮比例(g∶g)17∶3．在此条件下，糖化酶活力达1,641.69,U/g，酸性蛋白酶活力达151.09,U/g．同时研究了NaCl含量对红曲霉酶活力的抑制作用，当NaCl质量分数达到18.92%时，红曲霉MQ7产糖化酶活力下降64.8%，酸性蛋白酶活力下降85.6%．
종8주홍곡매중사선출홍곡매MQ7작위고산당화매화산성단백매적공시균주，채용고태발효방법측정재불동발효온도、저물료액비、대미화부피비례조건하적산매정황．이용정교실험우화고태발효산매과정，학정해균주산매최가발효조건위：발효온도30,℃，저물료액비(g∶mL)20∶25，대미부피비례(g∶g)17∶3．재차조건하，당화매활력체1,641.69,U/g，산성단백매활력체151.09,U/g．동시연구료NaCl함량대홍곡매매활력적억제작용，당NaCl질량분수체도18.92%시，홍곡매MQ7산당화매활력하강64.8%，산성단백매활력하강85.6%．
Monascus MQ7 strain was obtained from eight Monascus strains as the highly glucuoamylase and acid protease producing strain. The solid-state fermentation conditions for the glucoamylase and acid protease production with Monascus MQ7 were studied. Based on single factor tests,in different fermentation temperature,substrate solid-liquid ratio,proportion of rice and bran,the glucoamylase and acid protease production conditions were optimized with L9(34)orthogonal test. The results are as follows:the fermentation temperature is 30,℃,substrate solid-liquid ratio(g∶mL)20∶25,and the proportion of rice to bran(g∶g)17∶3. Under the above conditions,the glucoamylase activity reached 1,641.69,U/g,and the highest acid protease activity reached 151.09,U/g. The inhibition of NaCl on enzyme activity was also verified. When the NaCl mass fraction reached 18.92%,Monascus MQ7,glucoamylase activity decreased 64.8%,and acid protease activity decreased 85.6%.