CAJ | 학술논문

针对目前肉桂酰胺工业生产采用的肉桂酸和二氯亚砜、浓氨水两步化学反应工艺存在安全性较低、产物分离纯化困难等问题，建立并优化了赤红球菌 CGMCC3090催化肉桂腈生成肉桂酰胺的转化工艺．研究表明：底物肉桂腈浓度为1.5,mol/L，赤红球菌菌体质量浓度为2.6,g/L，在30,℃、pH 7.5条件下，转化5,h后转化率可达100%，具有产物纯度高、无副产物肉桂酸生成的优势，为赤红球菌CGMCC3090生物转化合成肉桂酰胺的工艺放大和生产性应用奠定了良好基础．
침대목전육계선알공업생산채용적육계산화이록아풍、농안수량보화학반응공예존재안전성교저、산물분리순화곤난등문제，건립병우화료적홍구균 CGMCC3090최화육계정생성육계선알적전화공예．연구표명：저물육계정농도위1.5,mol/L，적홍구균균체질량농도위2.6,g/L，재30,℃、pH 7.5조건하，전화5,h후전화솔가체100%，구유산물순도고、무부산물육계산생성적우세，위적홍구균CGMCC3090생물전화합성육계선알적공예방대화생산성응용전정료량호기출．
At present,the method of cinnamamide production consists of two steps by using cinnamic acid,thionyl chloride and strong ammonia water. This chemical process has less security,product separation and purification are difficult and there are some other problems. To solve these problems,the process of biotransformation of cinnamonitrile into cinnamamide us-ing Rhodococcus ruber CGMCC3090,was established. It was found that the conversion after 5,h can reach 100%when the cinnamonitrile was 1.5,mol/L,the cell concentration was 2.6,g/L,the temperature 30,℃ and pH 7.5. This process has the advantage of high product purity without formation of cinnamic acid. It has laid a good foundation for the scale-up process and practical production of cinnamamide biosynthesis with R. ruber CGMCC3090.